Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 111)

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MAP Technology Troubleshooting
PN 89-00002-00-150 Rev. A 8 - 9
Acquisition
Problems
Beads were left in the system
after Classification calibration. Perform an Alcohol Flush
followed by a Wash command.
Zero events collected during
calibration There is a problem with fluid
levels. Check the fluid levels in the
sheath and waste containers.
Verify that both bottles are
tightly connected to the
instrument. Check that the waste
bottle cap is vented.
Symptom Possible Problem Solution
Acquisition fails or slows The air pressure is out of range. See Pressurization on page 8-3.
The sample probe is not
vertically aligned. Adjust the sample probe height.
See page 7-9.
The sample probe is clogged. Clean sample probe. See
page 7-4.
The sheath bottle has a leaky
seal. Make sure that the sheath bottle
lid is tightened. Remove and
replace the sheath bottle lid.
The sheath or waste lines are
not fully connected. Check to make sure all tubes are
tightly connected
The Calibration microspheres
have expired. Replace old microspheres with a
fresh lot.
The calibration lot number
selected in setup is incorrect. In the Main Window of the
Luminex Data Collector
software, click Calibrate. In the
Start Calibration dialog box,
make sure that the correct lot
numbers are shown in the
Calibration Channel menu.
Target values for chosen
calibration lot number are
incorrect. Open the Start Calibration
dialog box. Highlight the lot
number, then click Delete. Re-
enter the calibration lot
information.