Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 13)

PN 89-00002-00-150 Rev. A 1 - 1
Introduction
The Luminex ® 200™ system is a compact analysis unit consisting of
a Luminex analyzer, the Luminex XY Platform (Luminex ® XYP™),
a PC, Luminex software, and reagents. The Luminex analyzer
integrates optics, fluidics, and advanced signal processing in a 17
inch by 20 inch footprint. The Luminex XYP provides efficient
handling of 96-well microtiter plates. The PC uses Luminex software
to control the analyzer. The optional Luminex Sheath Delivery
system (Luminex ® SD™) is used to provide sheath fluid directly
from a 20 liter container.
Photodiodes and a photomultiplier tube receive fluorescent signals
from xMAP ® microspheres. The analyzer digitizes the waveforms
and delivers the signals to a digital signal processor (DSP).
Proprietary algorithms function with the DSP to greatly increase the
sensitivity.
Hardware and preloaded software simplify installation and setup, and
with the help of this manual, you will be able to begin using xMAP
technology.
Principle of
Operation
The Luminex 200 system analyzes immunoassays, complex genetic
analyses, and enzymatic assays in one simple format. The reactants
(antibodies, oligonucleotides, substrates, etc.) of these assays are
anchored to the surfaces of uniquely fluorescent microscopic beads,
called microspheres. The unique fluorescent emission spectra of a
given microsphere identifies each of the assays performed
simultaneously on a single sample.
The Luminex analyzer individually analyzes xMAP microspheres in
a flow stream. Every xMAP microsphere is accurately classified to
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