Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 129)

x
MAP Technology Protocols
PN 89-00002-00-150 Rev. A 9 - 11
9. Add 500 µl of BLOCKING/STORAGE BUFFER to the
microspheres and vortex. Centrifuge the tube for 60 seconds.
Aspirate the supernatant.
10. Repeat step 9.
11. Resuspend the microspheres in the desired volume of
BLOCKING/STORAGE BUFFER.
12. Enumerate the microsphere preparation.
13. Store the preparation at 2
o
C - 8°C. Protect it from light.
Technical notes
1. The concentration of Biotin-conjugated molecules is provided as
a guide for assay development. The concentration required for
optimal reactivity must be determined empirically for each
specific biotin-conjugated molecule.
2. The reaction volume can vary with the reagent volume. If the
reagent is limited, perform the reaction in a proportionately low
volume.