YSI 9600 - Manual (Page 22)

Now proceed to the installation of the reagent bags as described in Section 2.7 below.
2.7 INSTALLING THE REAGENT BAGS
The next step in setting up the Model 9600 for field use is to install the six 1-liter reagent bags containing
solutions prepared in Section 2.4 above into the nitrate monitor carousel. Remove the carousel from the
outer shell of the analyzer and place it on the floor or a bench top in the vertical position supported by the
three legs. Then follow the step-by-step instructions below to install the reagent bags.
• Locate the six lines of reagent tubing which have color-coded threaded fitting on the ends. The ends
of the tubing with threaded fittings will be found hanging below the middle plate of the carousel and
should be retrieved manually. Note that these are the only loose fittings within the carousel, so their
identification should not be a problem.
• Note that there are three compartments in the top section of the carousel and that each compartment
must hold two reagent bags. The bags will be placed on the hooks that are hanging from the top plate
of the reagent compartments.
• Retrieve one of the fittings which is hanging in the carousel, note its color and then locate the filled
reagent bag with the same colored tie-wrap on its middle port. Make sure that each bag has the proper
reagent as shown in the table below. As long as the color code was followed in the filling of the
reagent bags, no possibility of error exists at this step. Make sure that the recovered tubing is routed
through the hole in the middle of the bottom plate rather than around the edges.
REAGENT BAG COLOR
Standard – 2 bags White
Blank – Purified Water Black
Buffer Blue
NED Red
Sulfanilimide Yellow
Wash – Purified Water Green
• Make the tubing to reagent bag connection by screwing the threaded fitting on the tubing into the
middle port of the reagent bag. Tighten securely with fingers only – no tools should be used.
• Hang the connected bag from one of the six hooks in the upper plate of the carousel.
• Repeat the sequence for the remaining five reagent bags making sure that there are two bags hanging
in each compartment, one on each hook.
• Open the pinch clamps on all bags and flick the middle port of each of the bags with your finger to
cause any remaining air bubbles to move to the top (closed end) of the reagent bags as shown in the
picture below.
YSI Environmental 22 Model 9600 Nitrate Monitor