YSI 9600 - Manual (Page 18)

The reagent bag is now ready for installation in the nitrate analyzer.
Repeat the filling process for all six reagent bags. Note that the wash (green fitting) and blank (black
fitting) bags should be filled with purified water only.
NOTE: Be sure to rinse the 60 mL syringe with purified water before switching reagents to avoid cross
contamination in the reagent bags.
2.5 PREPARING THE CADMIUM COLUMN
The cadmium column that was set aside during the unpacking process in Section 2.1 is a key ingredient in
the Model 9600 function and MUST be activated prior to use in the nitrate monitor. Activation should be
carried out according to the instructions below.
CAUTION: Cadmium, according to the USEPA, is toxic and a probable human carcinogen. Be sure
to handle the column and perform the activation process in an approved fume hood. Be sure to
wear appropriate personal protective equipment including eye protection and wear gloves while
handling the column and performing the activation process. Failure to observe these precautions
may lead to potentially serious health problems.
To activate the cadmium column, you must locate the 10-mL plastic syringe assembly that is part of the
9615 Syringe Kit that was supplied with the nitrate monitor. Note that the syringe is fitted with tubing and
a threaded fitting. This syringe is used to push reagents through the cadmium column during the activation
process. You will also require the following reagents that were prepared or purchased above:
• Hydrochloric Acid (10 % v/v)
• Ammonium Chloride Buffer Solution
• Copper Sulfate Solution (10 % w/v)
Once the reagents and the 10 mL syringe assembly have been located, you are ready to proceed to the next
step.
2.5.1 PREPARING THE COPPER SULFATE REAGENT
The copper sulfate solution (10 % w/v), purchased as described in 2.3.1 above, MUST be diluted prior to
its use in cadmium column activation. Perform the dilution by placing 2 mL of the purchased 10 % (w/v)
YSI Environmental 18 Model 9600 Nitrate Monitor