YSI 9600 - Manual (Page 19)

reagent in a beaker or other suitable container and adding 8 mL of purified water. The resulting 2 % (w/v)
solution will be used as part of the cadmium column activation process.
2.5.2 CADMIUM COLUMN ACTIVATION
CAUTION: In case an inadvertent leak occurs during the following procedure, it is prudent to
perform all operations with the apparatus placed in a flat glass baking dish with dimensions of about
9” x 18 “ x 1”. This procedure allows all leaks to be contained and allows for proper cleanup and
disposal of any spilled reagents.
• Working in an approved fume hood, open the box and remove the cadmium column assembly – shown
in the picture below.
• Wearing appropriate protective equipment, remove the threaded plugs at the ends of the column
assembly and set aside for later reassembly.
• Place about 15 mL of 10 % (v/v) hydrochloric acid in a beaker or other suitable vessel. With the
syringe plunger fully depressed, place the threaded fitting at the bottom of the syringe assembly in the
reagent and withdraw 10 mL into the syringe by pulling back on the plunger.
• Connect the threaded connector on the syringe assembly to one end of the cadmium column and
tighten by hand – no tools should be used. Mark the direction of flow that you have chosen through
the column by using a marker to place an arrow on the cadmium column assembly.
• Hold the open end of the cadmium column over a beaker or other suitable vessel and then depress the
plunger on the syringe assembly to push the acid steadily through the column as shown in the picture
below. The entire injection process should take approximately 15 seconds.
YSI Environmental 19 Model 9600 Nitrate Monitor