YSI 9600 - Manual (Page 14)

Ammonium Chloride – Fisher Scientific, Item Number A661-500
Sulfanilamide – Sigma , Item Number S-9251
N-(1-napthyl)ethylenediamine dihydrochloride (NED) – Aldrich, Item Number 22,248-8
Potassium Nitrate -- Alfa Aesar Chemical, Item Number 13443
Hydrochloric Acid (10 % v/v; 1.2 M) -- Fisher Scientific, Item Number LC15070-6 (10 liter container)
Copper Sulfate Solution (10 % w/v) -- Spectrum Chemical Mfg. Corp., Item Number C-283
BRIJ 35 Surfactant, Sigma -- 430AG-6
Kodak Photo-Flo 200 Wetting Agent -- Warehouse Photographic, Carrollton, TX, 800-400-8203,
www.warehousephoto.com, 146-4502. Alternatively, this chemical can be purchased at most camera
stores or photo supply houses.
2.3.2 REQUIRED EQUIPMENT AND CONTAINERS
Before proceeding with solution preparation, YSI recommends that you first make sure that you have
available the necessary equipment and storage vessels. First, you should have the proper bottles for storage
of at least 1.5 liters of each reagent. The plastic bottles recommended by YSI for this purpose are 2 liters
in capacity and are graduated so that 1.5 liters of solution can be prepared to the required accuracy for most
of the reagents. These bottles can be purchased from Fisher Scientific in packs of 6 or 12 as Fisher Item
Number 03-311-3g. Second, for preparation of the nitrate standards in various concentrations, you will
need a 1000 mL glass volumetric flask to obtain the desired accuracy. Third, users will require an
adjustable volume pipet for the 0 to 1 mL range such as the Brinkman Eppendorf model which can be
ordered from Fisher Scientific under Item Number 05-402-50. Fourth, if you wish to prepare nitrate
standards above 2 mg/L, you will need glass pipets with the appropriate volumes. For example, to prepare
a 5 mg/L nitrate standard, you will need a 5 mL glass pipet. Finally, users will require a balance that will
weigh accurately to at least 0.01 gram and preferably one which has an accuracy of 0.001 gram. In
summary, the following parts are required prior to reagent preparation:
• Graduated Plastic Bottles with 2 L capacity (at least 6)
• Volumetric Flask for 1000 mL (at least 1)
• Adjustable pipetter for 0-1 mL volumes (at least 1)
• Glass pipets of various denominations (depending on standard concentrations required)
• Laboratory balance with weighing accuracy of 0.01 g
2.3.3 SOLUTION PREPARATION
The following instructions should be followed to prepare the aqueous solutions that will be required for use
with the 9600 Nitrate Monitor. At least 1.5 L of the reagents and 3 L of the final standard should be
prepared in order to have enough solution volume to set up the nitrate monitor properly for a deployment.
CAUTION: Be sure to consult the MSDS for each chemical before proceeding and use safe
laboratory practices as outlined above during their handling.
BUFFER – Weigh 128 g of ammonium chloride to the nearest gram and place the solid in a 2 L graduated
plastic bottle. Add 1.5 L of purified water (distilled or deionized) and stir or shake until the solid is
completely dissolved. Using the adjustable pipetter, add 75 uL of 10 % copper sulfate solution and shake
well to mix.
YSI Environmental 14 Model 9600 Nitrate Monitor