Bio-Rad Precision Melt Analysis™ Software - Manual (Page 9)

HRM Analysis
4
• Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ (2003). High resolution
genotyping by amplicon melting analysis using LCGreen. Clin Chem. 49(6):853-60.
• Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E, Wittwer C (2004).
Genotyping of single-nucleotide polymorphisms by high-resolution melting of small
amplicons. Clin Chem. 50(7):1156-64.
• Vossen RH, Aten E, Roos A, den Dunnen JT (2009). High-resolution melting analysis
(HRMA): more than just sequence variant screening. Hum Mutat, 30:860-866.
Advanced Techniques
• Seipp MT, Durtschi JD, Liew MA, Williams J, Damjanovich K, Pont-Kingdon G, Lyon
E, Voelkerding KV, Wittwer CT (2007). Unlabeled oligonucleotides as internal
temperature controls for genotyping by amplicon melting. J Mol Diagn. 9(3):284-9.
• Dames S, Margraf RL, Pattison DC, Wittwer CT, Voelkerding KV (2007).
Characterization of aberrant melting peaks in unlabeled probe assays. J Mol Diagn.
9(3):290-6.
SNP Detection
• Temesvari M, Paulik J, Kobori L, Monostory K (2011). High-resolution melting curve
analysis to establish CYP2C19*2 single nucleotide polymorphism: Comparison with
hydrolysis SNP analysis. Mol Cell Probes, 25:130-133.
• Garritano S, Gemignani F, Voegele C, Nguyen-Dumont T, Le Calvez-Kelm F, De Silva
D, Lesueur F, Landi S, and Tavtigian SV (2009). Determining the effectiveness of High
Resolution Melting analysis for SNP genotyping and mutation scanning at the TP53
locus. BMC Genet, 10: 5.
Oncology (heterozygous dominant mutations & allele fractions)
• Willmore-Payne C, Holden JA, Chadwick BE, Layfield LJ (2006). Detection of c-kit
exons 11- and 17-activating mutations in testicular seminomas by high resolution
melting amplicon analysis. Mod Path. 19(9):1164-9.
Infectious Disease (haploid genomes)
• Dames S, Pattison DC, Bromley LK, Wittwer CT, Voelkerding KV (2007). Unlabeled
probes for the detection and typing of herpes simplex virus. Clin Chem. 53(10):1847-
54.
• Slinger R, Bellfoy D, Desjardins M, Chan F (2007). High-resolution melting assay for
the detection of gyrA mutations causing quinolone resistance in Salmonella enterica
serovars Typhi and Paratyphi. Diagn Microbiol Infect Dis 57:455-458.
HLA Matching
• Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais R, Wittwer CT (2004). High-
resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue
Antigens. 64(2):156-64.
Genotyping for Genetic Disorders
• Poulson MD, Wittwer CT (2007). Closed-tube genotyping of apolipoprotein E by
isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting
analysis. Biotechniques. 43(1):87-91.
• Seipp MT, Pattison D, Durtschi JD, Jama M, Voelkerding KV, Wittwer CT (2008).
Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by
high-resolution amplicon melting. Clin Chem. 54(1):108-15.
Mutation Scanning for Genetic Disorders