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Reports for melt files, D(rfu)/dt spreadsheet – Bio-Rad Precision Melt Analysis™ Software User Manual

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Analyzing Melt Data

50

Table 25 lists the type of information shown in the RFU spreadsheet.

-d(RFU)/dT Spreadsheet

Select the -d(RFU)/dT spreadsheet to view the type of data shown in Figure 42.

Figure 42. The -d(RFU)/dT spreadsheet in the Melt Curve Data tab.

Reports for Melt Files

The Report window (Figure 43) shows information about the current melt file in the Data
Analysis window. To create a report, select Tools > Reports, or click Reports on the toolbar in
the Data Analysis window.

The Report window shows four sections:

Menu and toolbar. Select options to format, save, and print the report or template

Options list (top, left side of window). Select data options and run settings to show in
the report

Options pane (bottom, left side of window). Enter information about a selected option

Table 25. RFU spreadsheet content

Information

Description

Well number (A1, A2, A3, A4,
A5...)

Well position in the plate for the loaded wells

Temperature

Melting temperature of the amplified target. Plotted as one
well per row, and multiple wells for multiple products in the
same well