Bio-Rad Precision Melt Analysis™ Software - Manual (Page 43)

Analyzing Melt Data
38
M ELT CURVE S HAPE S ENSITIVITY
Clustering shape sensitivity determines the stringency used to classify melt curves into
different clusters. To refine clustering results, increase or decrease the sensitivity of clustering
based on the shape of the melt curve. Enter a numerical value or move the slider bar to the left
or to the right. Entering a lower percentage value or sliding the bar to the left reduces
stringency and results in less heterozygote clusters. Entering a higher percentage value or
sliding the bar to the right increases stringency and results in more heterozygote clusters.
If necessary, manually decrease sensitivity to eliminate high numbers of false positives. A high
sensitivity value generally produces more groups than a low value. For most applications, the
default 50% clustering shape sensitivity value produces acceptable results.
T
M
DIFFERENCE T HRESHOLD
T
m
difference threshold determines the lowest amount of T
m
difference between samples
which the software will call as different clusters. Choose the number of degrees Celsius by
entering a numerical value from 0.05 to 1.00. Lower values produce more homozygote
clusters.
If necessary, manually increase the T
m
difference threshold level to eliminate false positives.
For most applications, the default T
m
difference threshold of 0.15 degrees produces
acceptable results.
Analysis Settings Profiles
Click Load from Analysis Options Manager to select a previously saved analysis settings
profile from the Load Analysis Options window. Click OK to apply the analysis settings.
Click Save to Analysis Options Manager to save the analysis options settings currently
selected in the Analysis Options Manager. Enter a name for the settings profile in the text box
in the Save Analysis Options window and click OK.