Bio-Rad Precision Melt Analysis™ Software - Manual (Page 54)

Precision Melt Analysis Software Instruction Manual
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Plate Spreadsheet
Select the Plate spreadsheet (Figure 40) to view melt curve data in a plate format.
Figure 40. Plate spreadsheet in Melt Curve Data tab.
NOTE: To adjust the peak that the software calls, adjust the threshold line in the
Melt Peak chart on the Melt Curve tab.
The Plate spreadsheet includes the types of information shown in Table 24.
RFU Spreadsheet
Select the RFU spreadsheet to view the fluorescence for each well at each cycle acquired
during the melt curve (Figure 41).
Figure 41. RFU spreadsheet in Melt Curve Data tab.
Table 24. Plate spreadsheet contents
Information Description
Content A combination of Sample Type (required) and Replicate #
(optional)
Sample Sample description
Peak 1 First melt peak (highest)
Peak 2 Second (lower) melt peak