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Normalized rfu spreadsheet – Bio-Rad Precision Melt Analysis™ Software User Manual

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Precision Melt Analysis Software Instruction Manual

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The raw RFU spreadsheet includes the type of information shown in Table 19.

Normalized RFU Spreadsheet

Select the Normalized RFU spreadsheet to view normalized fluorescence readings for each
well. Negative wells do not appear in this spreadsheet because they are excluded from
normalized processing (Figure 36).

Figure 36. Precision Melt data tab showing the Normalized RFU data

The Normalized RFU spreadsheet includes the type of information shown in Table 20.

Table 19. Raw RFU spreadsheet contents

Information

Description

Well number
(A2, A3, A4, A5, A6...)

Well data, listed by position in the plate for all the loaded wells

Temperature

Temperature data listed in ascending order for all the loaded
wells

Table 20. Normalized RFU spreadsheet contents

Information

Description

Well number
(A2, A3, A4, A5, A6...)

Well data, listed by position in the plate for all the loaded wells

Temperature

Temperature data listed in ascending order for all the loaded
wells