Normalized rfu spreadsheet – Bio-Rad Precision Melt Analysis™ Software User Manual
Page 50
Precision Melt Analysis Software Instruction Manual
45
The raw RFU spreadsheet includes the type of information shown in Table 19.
Normalized RFU Spreadsheet
Select the Normalized RFU spreadsheet to view normalized fluorescence readings for each
well. Negative wells do not appear in this spreadsheet because they are excluded from
normalized processing (Figure 36).
Figure 36. Precision Melt data tab showing the Normalized RFU data
The Normalized RFU spreadsheet includes the type of information shown in Table 20.
Table 19. Raw RFU spreadsheet contents
Information
Description
Well number
(A2, A3, A4, A5, A6...)
Well data, listed by position in the plate for all the loaded wells
Temperature
Temperature data listed in ascending order for all the loaded
wells
Table 20. Normalized RFU spreadsheet contents
Information
Description
Well number
(A2, A3, A4, A5, A6...)
Well data, listed by position in the plate for all the loaded wells
Temperature
Temperature data listed in ascending order for all the loaded
wells