Bio-Rad Precision Melt Analysis™ Software - Manual (Page 42)

Precision Melt Analysis Software Instruction Manual
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Analysis Options Window
Select Analysis Options from the toolbar of the Data Analysis window. In the Analysis Options
window, refine clustering results by increasing or decreasing the stringency used to classify
well data into different clusters (Figure 27).
Figure 27. Analysis Options manager window.
Choose the settings for analyzing melt file data in the Data Analysis window. After making
changes to the settings, click OK to return to the Data Analysis window and apply those
changes. Click Cancel to close the Analysis Options window without making any changes to
the currently applied settings.
• Auto detect the melt region. Select the checkbox to enable the software to
automatically define pre-melt and post-melt temperature ranges. To manually define the
pre-melt and post-melt ranges, deselect the checkbox and enter temperatures values by
typing a number in the text box or by using the up and down arrows
• Normalized view. Select to view the melt curve data displayed in normalized view in the
Melt Curve chart
• Temperature shifted view. Select to apply a temperature shift to each normalized
fluorescence curve along the temperature axis (x-axis)
• Temperature shift bar height. Specify the temperature shift bar height by entering a
number between 0 (baseline) and 1 (top of melt region). For most applications, the
default temperature shift bar height of 0.20 produces acceptable results, with the melt
curves clustered into tight groups
Cluster Detection Settings
Set the cluster detection settings to determine the stringency used to cluster the melt curves.