Bio-Rad Precision Melt Analysis™ Software - Manual (Page 47)

Analyzing Melt Data
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Precision Melt Data Tab
The Precision Melt Data tab shows spreadsheets that describe the melt data collected in each
well. Select one of the six options to show the data in different formats.
• Results. Displays a spreadsheet view of the data
• Charts. Displays multiple data charts in a single sheet
• Plate view. Displays a view of the data in each well as a plate map
• Raw RFU. Displays the RFU value in each well for each temperature
• Normalized RFU. Displays the normalized RFU value in each well for each temperature
• Difference RFU. Displays the difference RFU value in each well for each temperature
TIP: Right-click any spreadsheet for options. Sort the data in any spreadsheet by
right-clicking and choosing the Sort option. Click View/Edit Plate to open the
Plate Editor, and change the contents of any wells in the plate.
Results Spreadsheet
Select a Results spreadsheet (Figure 32) to see data for each well in the plate.
Figure 32. Precision Melt Data tab with Results spreadsheet selected.
The Results spreadsheet includes the information listed in Table 16.
Table 16. Results spreadsheet contents
Information Description
Well Well position in the plate
Content A combination of the Sample Type and Replicate # loaded in
the well in the Plate Editor
Sample Sample name loaded in the well in the Plate Editor
Cluster Name of the cluster assignment for the well
Percent Confidence Confidence percent value as an integrity check for auto-called
results
Call Type Automatic or Manual. Automatic means the software assigned
the cluster call for the well. Manual means the user assigned
the cluster call for the well