Waters SunFire Columns - Manual

[ Care and Use ManUal ]
Contents
I. GettInG Started
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
II. Column uSe
a. Guard Columns
b. Sample Preparation
c. pH Range
d. Solvents
e. Pressure
f. Temperature
III. SCalInG uP/doWn ISoCratIC metHodS
IV. trouBleSHootInG
V. Column CleanInG, reGeneratInG and StoraGe
a. Cleaning and Regenerating
b. Storage
VI. ConneCtInG tHe Column to tHe HPlC SyStem
a. Column Connectors and System Tubing Considerations
b. Bandspreading Minimization
c. Measuring System Bandspreading Volume & System Variance
d. Measuring System Volume
VII. addItIonal InFormatIon
a. Use of Narrow-Bore (≤3.0 mm i.d.) Columns
b. Impact of Bandspreading Volume on 2.1 mm i.d.
Column Performance
c. Non-Optimized vs. Optimized LC/MS/MS System:
System Modification Recommendations
Thank you for choosing a SunFire ™ column. The SunFire packing
materials were designed to provide excellent peak shape, minimal
column bleed, and high mass loading. The SunFire packing materials
are manufactured in an ISO 9000 certified plant using ultra pure
reagents. Each batch of SunFire material is tested chromatographi-
cally with acidic, basic and neutral analytes and the results are held
to narrow specification ranges to assure excellent, reproducible
performance. Every column is individually tested and a Performance
Chromatogram is provided with each column along with the Certificate
of Batch Analysis.
SunFire Column Physical Characteristics
Chemistry C
18
C
8
Silica
USP Class No. L1 L7 L3
Particle Shape Spherical Spherical Spherical
Particle Sizes
2.5, 3.5,
5, 10 µm
2.5, 3.5,
5, 10 µm
5, 10 µm
Pore Size 100Е 100Е 100Е
Surface Area 340 m 2 /g 340 m 2 /g 340 m 2 /g
Carbon Load 16% 12% N/A
Endcapped Yes Yes N/A
ph Range 2-8 2-8 N/A
Temperature
Limits Low pH = 50 ˚C
High pH = 40 ˚C
Low pH = 40 ˚C
High pH = 40 ˚C
N/A
sunFire Columns