Waters Envirogel GPC Cleanup Column - Manual (Page 5)

[ Care and Use ManUal ]
5 Waters Envirogel GPC Cleanup Column
Due to variations in detector sensitivities and cell volumes, you
may need to dilute the calibration solution to achieve the specified
resolution.
Refer to the Waters GPC Cleanup System Operator’s Guide,
Chapter 3 “Calibrating the System” for information on setting frac-
tion collection times.
iii. Column Care anD use guiDelines
Gel permeation chromatography columns have a finite lifetime
directly related to their care and use. Column life is reduced
by contamination from samples and eluents, frequent solvent
changeover, and improper handling and storage.
For best results, observe the following guidelines:
• Protect the column from vibration and mechanical shock.
• Do not exceed flow rates of 9 mL/min or pressures greater than
1000 psi.
• Do not use mobile phases which include water, ketones, alkanes,
or alcohols (except hexafluoroisopropanol).
• Protect the column from rapid changes in pressure, which can
result from rapidly changing the flow rate of the solvent. Change
flow rates in increments no more than 11 mL/min each minute.
• Dedicate columns to a specific application. Frequently switching
samples and solvents accelerates column deterioration and loss
of resolution.
• Be sure that columns do not dry out. Replace the end plugs when
storing the columns.
iv. Column storage
Refer to the Waters GPC Cleanup System Operator’s Guide for
system shutdown and storage procedures.
a. Short-term
During short-term column storage (storage overnight or a weekend),
ensure that columns do not dry out. Leave the system power ON, and
reduce the flow rate to the column to 0.5 mL/min. Divert the detector
output to waste. Recalibrate the system upon startup.
b. Long-term
Follow the long-term shutdown procedure in the Waters GPC
Cleanup System Operator’s Guide. Return the column to its box with
the end plugs firmly in place. Store the column at room temperature.