Waters Envirogel GPC Cleanup Column - Manual (Page 4)

[ Care and Use ManUal ]
4 Waters Envirogel GPC Cleanup Column
c. Injecting the Calibration Solution
To inject the calibration solution:
1. Filter approximately 10 mL of calibration solution through a GHP
Acrodisc 0.45 μm filter.
2. For 717 autosamplers, fill a WISP sample vial with 3 to 4 mL
of calibration solution, and place the vial in the carriage at
position 1.
3. For manual injectors with 2 mL sample loop, fill a 10 mL syringe
with calibration solution. Inject 2 mL of solution into the sample
loop.
4. Inject the calibration solution by either:
• Pressing the RUN/STOP key on the autosampler, or
• Switching the injector to INJECT
The initial peak for corn oil appears approximately 10 to 12 minutes
after injection. The final peak for sulfur appears approximately
25 minutes after injection. Figure 2 shows a sample calibration
chromatogram.
d. Calculating Peak Resolution
To calculate the resolution between the peaks, measure the
resolution height of the valley between the peaks, and the height of
the smaller peak (Figure 3).
Calculate the resolution by:
%R = 100 (1 - A/B)
Where:
R = resolution in percent
A = height of the valley
B = height of smaller peak
e. Requirements
EPA Method 3640A requires that:
•
All peaks are observed and symmetrical.
• The observed resolution between the following peaks is greater
than 85%:
• Corn oil and phthalate peaks
• Phthalate and methoxychlor peaks
• Methoxychlor and perylene peaks
• The observed resolution between the perylene and sulfur peaks
is greater than 90%.
Figure 2. Sample Chromatogram
Figure 3. Peak Resolution.