Waters Spherisorb Columns - Manual

[ Care and Use ManUal ]
Waters Spherisorb Columns 1
Waters spherisorb columns
contents
i. GettinG started
a. Column Installation
1. Reversed-Phase Columns
2. Normal-Phase Columns
b. Column Equilibration
1. Reversed-Phase Columns
2. Normal-Phase Columns
c. Initial Column Efficiency Determination
ii. column use
a. Guard Columns
b. Sample Preparation
c. pH Range
d. Solvents
e. Pressure
f. Temperature
iii. scalinG up/doWn isocratic methods
iv. troubleshootinG
v. column cleaninG, reGeneratinG and storaGe
a. Cleaning and Regenerating
1. Reversed-Phase Columns
2. Normal-Phase Columns
b. Storage
1. Reversed-Phase Columns
2. Normal-Phase Columns
vi. connectinG the column to the hplc
a. Column Connectors and System Tubing Considerations
b. Band Spreading Minimization
c. Measuring System Bandspreading Volume & System Variance
d. Measuring System Volume
vii. additional inFormation
a. Use of Narrow-Bore (≤3.0 mm i.d.) Columns
b. Impact of Bandspreading Volume on 2.1 mm i.d. Column Performance
c. Non-Optimized vs. Optimized LC/MS/MS System:
System Modification Recommendations
d. Guard Cartridges and Columns Assembly
Thank you for choosing a Waters Spherisorb ® column. Spherisorb
analytical columns are durable, high-efficiency chromatographic
columns, featured in thousands of references in chromatographic
literature. The wide range of column lengths and diameters offers
you exceptional flexibility in optimizing methods and reducing
solvent consumption. Follow the guidelines in this manual to obtain
the best performance, reproducibility and durability from your
analytical columns and cartridges.