Waters Symmetry Columns - Manual

[ Care and Use ManUal ]
Symmetry Columns 1
i. introduction
Thank you for choosing a Waters Symmetry ® column. Symmetry
columns continue to set the standard of performance for
reproducibility. As today’s chemists establish new analytical methods
for the latest pharmaceutical and biopharmaceutical products, the
selection of a reproducible HPLC column is essential. The selected
column needs to provide the same chromatographic results over the
life of the new drug product. The excellent reproducibility of Sym-
metry, SymmetryShield™ and Symmetry300™ columns is a result
of our commitment to maintaining the tightest specifications in the
HPLC column industry.
contents
i. introduction
ii. GettinG started
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
iii. column use
a. Guard Columns
b. Sample Preparation
c. pH Range
d. Solvents
e. Pressure
f. Temperature
iV. scalinG up/down isocratic methods
V. troubleshootinG
Vi. column cleaninG, reGeneratinG and storaGe
a. Cleaning and Regenerating
b. Storage
Vii. connectinG the column to the hplc
a. Column Connectors and System Tubing Considerations
b. Band Spreading Minimization
c. Cartridge Installation
d. Measuring System Bandspreading Volume & System Variance
e. Measuring System Volume
Viii. additional information
a. Use of Narrow-Bore (≤3.0 mm i.d.) Columns
b. Impact of Bandspreading Volume on 2.1 mm i.d. Column Performance
c. Non-Optimized vs. Optimized LC/MS/MS System:
System Modification Recommendations
d. Waters Small Particle Size (3.5 μm) Columns – Fast Chromatography
symmetry columns
Physical Characteristics
Packing Chemistry Particle Size Particle
Shape
Pore
Size
Carbon
Load
End-
capped
Symmetry C
18
C
8
3.5, 5 µm
3.5, 5 µm Spherical
Spherical 100Е
100Е 19%
12% Yes
Yes
SymmetryPrep C
18
C
8
7 µm
7 µm Spherical
Spherical 100Е
100Е 19%
12% Yes
Yes
SymmetryShield RP18
RP8
3.5, 5, 7 µm
3.5, 5, 7 µm Spherical
Spherical 100Е
100Е 17%
15% Yes
Yes
Symmetry300 C
18
C
4
3.5, 5 µm
3.5, 5 µm Spherical
Spherical 300Е
300Е 8.5%
2.8% Yes
Yes