Waters XSelect HSS XP 2.5 µm Columns - Manual (Page 7)

XSelect HSS XP 2.5 µm Columns 7
[ CARE AND USE MANUAL ]
b. Storage after Reversed-Phase Use
For periods longer than four days, store the XP 2.5 µm Column in
100% acetonitrile. For separations utilizing elevated temperature,
store immediately after use in 100% acetonitrile. Do not store
columns in buffered eluents. If the mobile phase contained a buffer
salt, flush the column with 10 column volumes of HPLC grade water
(see Table 3 for column volume information) followed by 10 column
volumes of acetonitrile. Failure to perform this intermediate step
could result in precipitation of the buffer salt in the column when
100% acetonitrile is introduced. Completely seal the column to avoid
solvent evaporation and drying out of the chromatographic bed.
Note: If a column has been run with a formate-containing mobile phase (e.g.,
ammonium formate, formic acid, etc.) and is purged with 100% acetonitrile,
slightly longer equilibration times may be necessary when the column is re-
installed and re-wetted with that same formate-containing mobile phase.
c. Storage after Normal-Phase Use
For rapid equilibration upon start-up, store the XP 2.5 µm Column in
a mobile phase that is commonly used for your normal-phase separa-
tion (i.e., hexane/isopropanol). Completely seal the column to avoid
solvent evaporation and drying out of the chromatographic bed.
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
The eCord ™ Intelligent Chip Technology represents a significant
advancement in column usage tracking management which can be
realized if the column is installed on an ACQUITY UPLC System.
The eCord Intelligent Chip provides a paperless tracking history of the
column’s performance and usage throughout its lifetime. The eCord
is permanently attached to the column body via a tether that cannot
be removed. This ensures that the history of the column is always
accessible to the user of that column.
Figure 5: eCord Intelligent Chip.
At the time of manufacture, information such as the performance test
chromatogram, column manufacturing QC data and Certificates of
Analysis is downloaded onto the eCord. This information may then be
accessed via the ACQUITY UPLC console once the column is installed.
b. Installation
The eCord device can be read by connecting the yellow fob to the
reader/writer located on the right-hand side of the ACQUITY UPLC
Column heater module. Once the eCord is connected to the magnetic
catch on the column heater, column identification and overall column
usage information can be accessed.
Heater compartment receptacle
eCord fob
Figure 6: Installing the eCord Intelligent Chip.