Waters XSelect HSS XP 2.5 µm Columns - Manual (Page 5)

XSelect HSS XP 2.5 µm Columns 5
[ CARE AND USE MANUAL ]
Note: VanGuard Pre-Columns are shipped with a collet and ferrule that are NOT
pre-swaged. This enables the end user to mate the VanGuard Pre-Column to
a specific XP 2.5 µm Column and ensures void-free and leak-free connections.
Care must be taken when removing the O-ring that holds these two pieces
on the pre-column tubing.
XP 2.5 µm Column VanGuard Pre-Column
Place wrench here
Ferrule
Collet
Place wrench here
Flow
Figure 4: Installing a VanGuard Pre-Column.
VanGuard Pre-Column Installation Instructions
1. Remove the VanGuard Pre-column from its box and shipping
tube and remove the plastic plug.
2. Orient the pre-column so that the male end is facing up and
carefully remove the black O-ring that holds the collet and
ferrule in place during shipment (collet and ferrule are not
permanently attached).
3. Orient the XP 2.5 µm Column perpendicular to the work surface
so that the column inlet is on the bottom.
4. From below, insert the VanGuard Pre-Column into the column
inlet; turn the assembled column and pre-column 180° so that
the pre-column is now on top.
5. Tighten with two 5/16” wrenches placed onto the XP Column
flats and VanGuard Pre-Column hex nut (male end) as shown in
Figure 4.
6. While keeping pressure on the VanGuard Pre-Column against the
XP 2.5 µm Column, tighten turn to set the collet and ferrule.
7. Check that the ferrule is set by loosening the connection and
inspecting the ferrule depth.
8. Reattach the pre-column to the XP 2.5 µm Column, apply flow
and inspect for leaks.
III. COLUMN USE
To ensure the continued high performance of XSelect HSS XP 2.5 µm
Columns, follow these guidelines:
a. Sample Preparation
1. Sample impurities and/or particulates often contribute to column
contamination. One option to avoid column contamination is to
use Waters Oasis
® or Sep-Pak ® Solid-Phase Extraction (SPE)
devices. To select the appropriate sorbent for a specific sample
type, visit www.waters.com/sampleprep
2. It is preferable to prepare the sample in the initial mobile-
phase conditions or a weaker solvent for the best peak shape
and sensitivity.
3. If the sample is not prepared in the mobile phase, ensure that
the sample, solvent and mobile phases are miscible in order to
avoid sample and/or buffer precipitation.
4. Filter sample with a 0.2 µm membrane to remove particulates.
If the sample is dissolved in a solvent that contains an organic
modifier (e.g., acetonitrile, methanol, etc.) ensure that the
membrane/filter material is compatible with the solvents
in use. Alternatively, centrifuge the sample for 20 minutes at
8000 rpm, followed by the transfer of the supernatant to an
appropriate vial could be considered.
b. pH Range
Table 4: Recommended pH Range
Chemistry pH Range
XSelect HSS Cyano 2 - 8
XSelect HSS PFP 2 - 8
XSelect HSS T3 2 - 8
XSelect HSS C
18
SB 2 - 8
XSelect HSS C
18
1 - 8
Column lifetime will vary depending on the combination of temperature,
mobile-phase pH and type of buffer/additive used. Table 5 lists the
recommended buffers and additives for XSelect HSS XP 2.5 µm Columns.
Note: Working in combinations of extreme pH, temperature and pressure may result in
reduced column lifetime.