Waters ACQUITY UPLC CSH130 C18 1.7 μm and XP 2.5 μm Columns - Manual (Page 5)

[ CARE AND USE MANUAL ]
ACQUITY UPLC CSH130 C
18
Columns 5
Table 3. Column Cleaning Sequence
Polar Samples Proteinaceous Samples
1. water Option 1: Inject repeated 100 µL aliquots of dimethylsulfoxide
(DMSO) using a reduced flow rate delivering 50% Eluent A and
50% Eluent B
2. methanol Option 2: gradient of 10% to 90% B where:
A = 0.1% trifluoroacetic acid (TFA) in water
B = 0.1% trifluoroacetic acid (TFA) in acetonitrile (CH
3
CN) 3. isopropanol
Option 3: Flush column with 7M guanidine hydrochloride,
or 7M urea
Note: To avoid potentially damaging precipitation within your column (e.g., if
your separation eluent contains phosphate buffer), be certain to flush column
with 5 to 10 column volumes of water BEFORE using suggested organic eluent
column wash procedures.
b. Storage
For periods longer than four days at room temperature, store the column in
100% acetonitrile. For elevated temperature applications, store immediately
after use in 100% acetonitrile for the best column lifetime. Do not store
columns in buffered eluents. If the mobile phase contained a buffer salt, flush
the column with 10 column volumes of HPLC grade water (see Table 1 for
common column volumes) and replace with 100% acetonitrile for storage.
Failure to perform this intermediate step could result in precipitation of the
buffer salt in the column when 100% acetonitrile is introduced. Completely
seal column to avoid evaporation and drying out of the bed.
Note: If a column has been run with a mobile phase that contains formate
(e.g., ammonium formate, formic acid, etc.) and is then flushed with 100%
acetonitrile, slightly longer equilibration times may be necessary when the col-
umn is re-installed and run again with a formate-containing mobile phase.
IV. eCORD
a. Introduction
The eCord intelligent chip is a new technology that will provide the history of a
column’s performance throughout its lifetime. The eCord is permanently attached
to the column to assure that the column’s performance history is maintained in the
event that the column is moved from one instrument to another.
Waters eCord intelligent chip
At the time of manufacture, tracking and quality control information will be
downloaded to the eCord. Storing this information on the chip will eliminate
the need for a paper Certificate of Analysis. Once the user installs the col-
umn, the software will automatically download key parameters into a column
history file stored on the chip. The eCord provides a solution to easily track
the history of column usage.
b. Installation
Install the column into the column heater. Plug the eCord into the side of the
column heater. Once the eCord is inserted into the column heater the identi-
fication and overall column usage information will be available in Empower
®
and MassLynx software allowing the user to access column information on
their desktop.
c. Manufacturing Information
eCord inserted into side
of column heater
The eCord chip provides the user
with QC test conditions and
results on the column run by the
manufacturer. The information
includes mobile phases, running
conditions and analytes used
to test the columns. In addition
the QC results and acceptance is
placed onto the column.