Waters ACQUITY UPLC CSH130 C18 1.7 μm and XP 2.5 μm Columns - Manual (Page 3)

[ CARE AND USE MANUAL ]
ACQUITY UPLC CSH130 C
18
Columns 3
d. eCord Installation
The eCord button should be attached to the side of the column heater module.
The eCord button is magnetized and does not require specific orientation.
e. Initial Column Efficiency Determination
1. Perform an efficiency test on the column before using it.
Waters recommends using a suitable solute mixture, as found in
the “Performance Test Chromatogram”, to analyze the column
upon receipt.
2. Determine the number of theoretical plates (N) and use this
value for periodic comparisons.
3. Repeat the test at predetermined intervals to track column per-
formance over time. Slight variations may be obtained on two
different UPLC systems due to the quality of the connections,
operating environment, system electronics, reagent quality,
column condition and operator technique.
f. VanGuard Pre-Columns
VanGuard ™ Pre-columns are 2.1 mm i.d. x 5 mm length guard column
devices designed specifically for use in the ACQUITY UPLC systems.
VanGuard Pre-columns are packed with the same chemistries and frits as our
2.1 mm i.d. ACQUITY UPLC CSH130 C
18
columns. VanGuard Pre-columns
are designed to be attached directly to the inlet side of an ACQUITY UPLC
CSH130 C
18
column.
Note: In order to ensure void-free and leak-free connections, the VanGuard
Pre-column is shipped with the collet and ferrule NOT permanently attached.
Care must be taken when removing the O-ring that holds these two pieces on
the pre-column tubing.
ACQUITY UPLC ® Column VanGuard ™ Pre-Column
Place wrench here
Ferrule
Collet
Place wrench here
Flow
Installation Instructions
1. Remove VanGuard Pre-column from box and shipping tube and
remove plastic plug.
2. Orient pre-column so that male end is facing up and carefully
remove rubber O-ring that holds collet and ferrule in place
during shipping (collet and ferrule are not yet permanently
attached).
3. Orient ACQUITY UPLC CSH130 C
18
column perpendicular to
work surface so that column inlet is on the bottom (column
outlet on top).
4. From below, insert VanGuard Pre-column into ACQUITY UPLC
CSH130 C18 column inlet and hand-tighten (collet and ferrule
are not yet permanently attached).
5. While pushing the VanGuard Pre-column into the column inlet,
turn assembled column and pre-column 180˚ so that pre-
column is now on top.
II. COLUMN USE
To ensure the continued high performance of ACQUITY UPLC CSH130 C
18
columns, follow these guidelines:
a. Sample Preparation
1. Sample must be dissolved in a diluent compatiable with initial
strength of mobile phase.
2. Sample must be completely in solution and free of particulates.
3. To remove particulates the sample may be filtered with a 0.2 µm
membrane. If the sample is dissolved in a solvent that contained
an organic modifier (e.g., acetronitrile, methanol, etc.) ensure that
the membrane material does not dissolve in the solvent. Contact
the membrane manufacturer with solvent compatibility questions.
Alternatively, centrifugation for 20 minutes at 8,000 rpm,
followed by the transfer of the supernatant liquid to an
appropriate vial, could be considered.