Waters ACQUITY UPLC CSH130 C18 1.7 μm and XP 2.5 μm Columns User Manual

Care and use manual, Acquity uplc csh130 c, 7 µm and xp 2.5 µm columns

[ CARE AND USE MANUAL ]

ACQUITY UPLC CSH130 C

Columns

Thank you for choosing a Waters ACQUITY UPLC

CSH

™

column.

ACQUITY UPLC CSH130 C

, 1.7 µm and XP 2.5 µm columns feature

Waters Charged Surface Hybrid (CSH) Technology which provides
excellent peak shape, high efficiency and loading capacity for basic
compounds when using acidic, low ionic strength mobile phases.
This same particle technology is used in the XSelect

™

CSH family of

HPLC columns, thus enabling seamless transferability between HPLC
and UPLC

system platforms. The ACQUITY UPLC CSH130 packing

materials were designed specifically for use with the ACQUITY UPLC
systems and are manufactured in a cGMP, ISO 9001 certified
manufacturing facility using ultra pure reagents. Each batch of
ACQUITY UPLC CSH130 C

material is tested chromatographically

with acidic, basic, and neutral analytes as part of qualification for
use in peptide mapping. The results are held to narrow specification
ranges to assure excellent, reproducible performance. ACQUITY UPLC
CSH130 C

batches are also QC tested with a gradient separation

of a tryptic digest of cytochrome c using 0.1% formic acid containing
eluents. Finally, every shipped column is individually tested for packed
bed efficiency and a Performance Chromatogram and Certificate of
Batch Analysis are provided on the eCord

™

intelligent chip. ACQUITY

UPLC CSH130 C

columns were designed and tested specifically for

use on ACQUITY UPLC systems. ACQUITY UPLC CSH130 C

columns

will exhibit maximum chromatographic performance and benefits ONLY
when used on holistically-designed ACQUITY UPLC systems since these
systems and columns were created and designed to operate together.
For these reasons, Waters cannot support the use of ACQUITY UPLC
columns on any system other than an ACQUITY UPLC system.

CONTENTS

I. GETTING STARTED

a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns

II. COLUMN USE

a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature

III. COLUMN CLEANING, REGENERATING AND STORAGE

a. Cleaning and Regeneration
b. Storage

IV. INTRODUCING ECORD INTELLIGENT CHIP TECHNOLOGY

V. REPRESENTATIVE TEST CHROMATOGRAPH AND CONDITIONS
FOR SEPARATION OF PROTEIN DIGEST

VI. ADDITIONAL INFORMATION

ACQUITY UPLC CSH130 C

1.7 µm AND XP 2.5 µm COLUMNS