Leica Biosystems PELORIS_PELORIS II - Manual (Page 78)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 78
5.1.4 Recommended Reagents
While each laboratory is responsible for its choice of reagents and wax, using reagents other than
those recommended for Peloris can result in poor processing or compromise instrument reliability.
Compliance with the following guidelines will ensure superior tissue processing.
The use of alternative reagents must be validated by the end user according to their local or
regional accreditation requirements.
Adequate fixation of tissue samples is required for optimum results and should be achieved prior to
placement on Peloris or incorporated into the fixative step of the protocol. The following reagents
have been validated for use on Peloris.
Fixatives

10% Neutral Buffered Formalin (NBF)
Alcohols

Histological grade ethanol

Reagent (grade) ethanol

Absolute ethanol

Isopropyl alcohol (dehydrant)
Denatured ethanol is acceptable if:

It is at least 99% ethanol, and

It is denatured with methanol and/or isopropyl alcohol (IPA)

Ethanol containing acetone must not be used.
Clearing Agents

Xylene is the recommended clearing agent.

Xylene-free processing requires the use of isopropyl alcohol as the clearing agent. It has been
fully tested and validated.
Paraffin

Use histological wax for histology

Parablocks™

Paraplast®
Cleaning Agents

Use ethanol for the cleaning alcohol solution.

In xylene processing mode, we recommend xylene as the cleaning solvent.

For true xylene-free processing, we recommend Waxsol™ as the cleaning solvent.