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0 troubleshooting – Bio-Rad EXQuest Spot Cutter User Manual

Page 76

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TROUBLESHOOTING

11-1

11.0 TROUBLESHOOTING

Symptom

Cause

Solution

Computer does not

USB cable not

Ensure that both the USB cable and power cord

communicate with

connected

are connected

spot cutter or
camera

Computer went into

Disable sleep mode on your computer (see

sleep mode

Section 2.9)

The spot cutter

Make sure that the power cord is connected

power switch has

and the power switch is turned on.

not been turned on.

Camera or spot cutter

Close the software and turn the spot cutter off.

are in an Error state

Turn the spot cutter back on, wait for approximately
30 seconds and then restart the software.

Poor image quality

Camera is not focused

Perform the camera focusing and camera
calibration steps described in Section 3.

F-stop set incorrectly

Perform the camera focusing and camera
calibration steps described in Section 3.

Low gel cutting efficiency

Empty water bottle

The spot cutter relies on water aspiration for
efficient gel pickup and deliver. Check the water
level and make sure that there is sufficient water.

Air bubbles

Large air bubbles in the tubing reduce the gel
pickup and delivery efficiency. Start manual excision
tool and click Prime Pump. Prime the system until
the air bubbles are removed.

Loose tubing

Loose fittings cause air to get into the fluidics

connectors

system. Make sure all fittings are tight

Tip Damage

Damaged or bent tips do not cut gels efficiently.
Examine the cutting tip for damage and replace it, if
necessary

Cutting sheet damage

Make sure that you are using the cutting sheet

(free gels)

supplied with the spot cutter. If it has been used
several times or is severely scarred, replace it. See
Section 9, Accessories for ordering information. Do
not remove the soft film from the cutting sheet, as it
is important for cutting efficiency.

Tip height adjustment

If the spot cutter is not cutting efficiently, recalibrate
the tip height as described in Section 3.

Gel hydration

Gels that become dry are not picked up efficiently. If
gels become dry, increase the hydration frequency.

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