Bio-Rad EXQuest Spot Cutter - Manual (Page 29)

Figure 3-9. System position calibration.
Manual Calibration
a. If calibrating the membrane cutting head, install the plastic protective sleeve over the 1.0 mm
cutting tip.
b. Click Next to start the system position calibration and move the cutting head to the first position, or
click Back to repeat the lens calibration step or Cancel to abort the calibration.
c. Center a puck under the cutting tip and then click Next to position the puck and move the cutting
head over the next puck, Back to reposition the previous puck or Cancel to abort the calibration.
d. Continue as described in Step b until all the pucks have been positioned.
e. Click Done and close the front door. The spot cutter will refine the puck placement, acquire an
image of the pucks, and then finish the calibration.
f. Remove the protective sleeve if the membrane cutting head was calibrated.
g. Remove the calibration pucks and store them in the box provided to prevent them from being damaged.
Automatic Calibration
a. If calibrating the membrane cutting head, install the plastic protective sleeve over the 1.0 mm
cutting tip.
b. Check the Automatically move pucks to alignment position box.
c. Click Next to perform an automatic puck placement. The spot cutter will take an image of the
pucks, move the pucks to the correct position, refine the puck placement, take another image and
then finish the calibration. Alternatively click Back to repeat the lens calibration or Cancel to abort
the calibration.
d. Remove the protective sleeve if the membrane cutting head was calibrated.
e. Remove the calibration pucks and store them in the box provided to prevent them from being damaged.
Once the System Position Calibration has been completed, it will automatically save the calibration. You may
now exit (Cancel) the calibration wizard without losing the calibration.
10. Plate Position Calibration. The plate position calibration step is used to center the cutting tip over the
microplate wells.
a. Place a microplate with the desired format in the back-right slot of the microplate rack with well A1
located in the front-left corner of the microplate. Select the microplate format and then click Next.
EXQUEST CALIBRATION WIZARD
3-7