Bio-Rad EXQuest Spot Cutter - Manual (Page 68)

EXCISION TOOL CONTROLS
8-4
• Material: Use this drop-down menu to select the gel type that will be cut, e.g., "Free gel", "Plastic-
backed gel" or "Glass-backed gel". When the membrane head is installed, "PVDF" will automatically
be selected.
8.5 PLATE OPTIONS PANEL
Figure 8-5. “Plate Options” panel
The "Plate Options" panel is used to set the microplate format, well volume and the well loading order. This
panel may appear in a tab box, as shown above, or as a separate panel.
• 96-well microtiter, 384-well microtiter, 96 tubes: Use these option buttons to select the plate format to
use.
• ml/well and ml/tube: These drop-down menus are used to identify the well volume for the microplate
format selected. Note that "ml/well" identifies the actual microplate well volume, whereas "Min. Well
Volume" in the “Run Info” panel (Section 8.8) is the minimum required well volume and is based on
the Maximum Cuts/object value and any pooling (Analysis Set Excision Tool advanced mode only)
performed as part of the run. The ml/well volume must be greater than or equal to Min. Well
Volume.
• Loading Order: Select (A1, A2 …) for a front-to-back fill order or (A1, B1 …) for a left-to-right fill
order.
8.6 HYDRATION OPTIONS PANEL
Figure 8-6. “Hydration Options” panel
The “Hydrate Options” panel is used to set up hydration parameters.
• Hydrate. Check this box to turn auto hydration on and to activate the hydration interval field. Enter
the hydration interval in minutes. The interval time should usually be set to approximately one hour.
The actual time required will depend on your laboratory temperature and humidity.