Bio-Rad EXQuest Spot Cutter - Manual (Page 63)

6. Adjust Horizontal slider to control the horizontal cut spacing. Use the zoom tool to see the effect of
changing the Horizontal control.
Figure 7-5. The effect of the Horizontal control on the cut requests: Left, Horizontal = 100;
Right, Horizontal = 0
7.3 CUTTING VOLUMES
Volume Overlay mode is used to select bands from which cuts will be made.
1. Select Volume overlay in the "Specify Cuts" panel.
Figure 7-6. Lane/Volume Excision Tool “Specify Cuts” panel, “Volume overlay” selected
2. Check the Make multiple cuts on large objects box to make multiple cuts from each band and
enter a value in the Maximum cuts/object field (see Section 8.4).
3. Click the Volume Rect Tool and then click and drag a box around the gel bands to cut. The box
size and the Maximum cuts/object value determines the number of cuts per band. To adjust the
box size: hold the cursor over one of the four corners until it changes to a two headed arrow cursor
and then click and drag the mouse. To move the box, place the mouse cursor over the box center
until it changes to a four-way arrow cursor and then click and drag the cursor. Check Show
excisions on image to show the cut request labels on the image.
LANE/VOLUME EXCISION TOOL
7-4