Bio-Rad EXQuest Spot Cutter - Manual (Page 25)

EXQUEST CALIBRATION WIZARD
3-3
Figure 3-4. “EXQuest Calibration Wizard” screen – calibrated.
From this screen you may
• Calibrate the camera with the current focus and aperture setting by clicking Next (Go to Step 6,
Head Height Calibration).
• Focus the camera and then calibrate it by checking Perform focus/alignment step and then
clicking Next (go to Step 5, Camera Focus).
• Skip the camera calibration steps by checking Skip to plate position step and then clicking
Next (go to Step 9, Plate Position Calibration).
5. Camera Focus. The Camera Focus dialog is used to monitor a camera image as the camera is
focused.
a. With the gel tray inserted in the spot cutter, place the calibration target on the gel tray and align
it with the left and front sides of the tray.
b. Locate the camera lens inside the spot cutter, above the door in between the UV lamps and
change the aperture (lower ring on the lens) to f-stop 6. To set the aperture to f-stop 6, turn the
lower ring all the way to the right (the front of the ring will move to the right) and then turn it to
the left 4 clicks (the front side of the ring will move to the left).
c. Adjust the focus (upper ring on the camera lens) while you monitor the image and the Focus
Levels field in the camera focus dialog. The Focus Levels will generally reach a minimum when
the camera is focused.
d. When finished, change the aperture to f-stop 12. To set the aperture to f-stop 12, turn the lower
ring all the way to the right (the front of the ring will move to the right) and then turn it to the left
2 clicks (the front side of the ring will move to the left). The camera focus is now set to the
optimum position. Click Done.