Bio-Rad EXQuest Spot Cutter - Manual (Page 41)

MANUAL EXCISION TOOL
5-6
Figure 5-6. Confirm cuts pop-up.
2. Read the instructions on the "Image Microtiter Plate" screen.
Figure 5-7. “Image Microtiter Plate” window.
3. Without moving the gel, place a clean plastic cutting sheet on top of the gel.
4. Place the microplates on top of the plastic sheet, note the orientation so you can recognize the A1
well location, and then close the door.
5. Click Acquire image to obtain an image with the same exposure time as the cut request image.
6. Closely examine the microplate wells in the image for the absence of gel plugs and note the well
position. Use the zoom and transform functions (see Section 8.3) on the right side of the "Image
Microtiter Plate" window to get a better view of the microplate wells.
7. Click Specify re-cuts to re-cut spots or No re-cuts if there are not any missed spots.
8. Click OK on the "Remove Microtiter Plate" pop-up box and then return each microplate to its original
location and orientation in the microplate rack. Carefully remove the cutting sheet from the top of the
gel so as to not move the gel.
9. If Specify re-cuts was selected in Step 7, click OK and then find the missed spot pickups on the gel
image from their well ID and right click on the spot to add it to the re-cut list.
10. Press OK to close the Re-cut request dialog.
11. Click Begin/Resume Cuts to perform the re-cuts.