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Bio-Rad EXQuest Spot Cutter User Manual

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TABLE OF CONTENTS

TABLE OF CONTENTS

SAFETY

SECTION 1

INTRODUCTION ..................................................................................................................1-1

1.1 Overview................................................................................................................................1-1
1.2 System Description................................................................................................................1-2

1.2.1

Gel Imaging System ................................................................................................1-2

1.2.2

Imaging System ........................................................................................................1-3

1.2.3

Gel Tray and Gel Clamps..........................................................................................1-3

1.2.4

Cutting Heads ..........................................................................................................1-4

1.2.5

Fluidics System ........................................................................................................1-5

1.2.6

Wash Solution ..........................................................................................................1-5

1.2.7

Microplate Rack ........................................................................................................1-6

1.2.8

Robotics....................................................................................................................1-7

1.2.9

Sensors ....................................................................................................................1-8

1.2.10 Front Panel Controls and Indicators ........................................................................1-8
1.2.11 Software....................................................................................................................1-9
1.2.12 Barcode Reader........................................................................................................1-9

SECTION 2

SYSTEM INSTALLATION ....................................................................................................2-1

2.1 Pre-Installation ......................................................................................................................2-1
2.2 Unpacking the EXQuest Spot Cutter ....................................................................................2-1
2.3 System Leveling ....................................................................................................................2-3
2.4 Gel Tray Installation ..............................................................................................................2-3
2.5 Microplate Rack Installation ..................................................................................................2-4
2.6 Cable Setup ..........................................................................................................................2-5
2.7 Cutting Head Installation ......................................................................................................2-6

2.7.1

Gel Cutting Head ......................................................................................................2-6

2.7.2

Membrane Cutting Head ..........................................................................................2-7

2.8 DI Water and Waste Bottle Installation ..................................................................................2-8
2.9 Software Installation ..............................................................................................................2-8
2.10 Starting the Software ............................................................................................................2-9

SECTION 3

EXQUEST CALIBRATION WIZARD ....................................................................................3-1

SECTION 4

GEL AND BLOT PREPARATION ........................................................................................4-1

4.1 Free Gels ..............................................................................................................................4-1
4.2 Plastic and Glass-Backed Gels ............................................................................................4-2
4.3 Blot Preparation ....................................................................................................................4-2

SECTION 5

MANUAL EXCISION TOOL. ................................................................................................5-1

5.1 Performing a Manual Excision Tool Cut Run ........................................................................5-1
5.2 Importing a Cut List ..............................................................................................................5-4
5.3 Confirm Cuts..........................................................................................................................5-5

5.3.1

Identifying Re-cuts From a Microplate Image ..........................................................5-5

5.3.2

Identifying Re-cuts From a Gel Image......................................................................5-7

SECTION 6

ANALYSIS SET EXCISION TOOL ......................................................................................6-1

6.1 Setting Up An Analysis Set Excision Run ............................................................................6-1
6.2 Starting A Cut Run ................................................................................................................6-6
6.3 Analysis Set Excision Tool – Advanced User Mode ............................................................6-13

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