Waters ACQUITY UPLC BEH Columns - Manual (Page 8)

c. Manufacturing Information
d. Column Use Information
The eCord chip provides the customer with column use data. The
top of the screen identifies the column including chemistry type,
column dimensions and serial number. The overall column usage
information includes the total number of samples, total number
of injections, total sample sets, date of first injection, date of last
injection, maximum pressure and temperature. The information
also details the column history by sample set including date
started, sample set name, user name, system name, number of
injections in the sample set, number of samples in the sample
set, maximum pressure and temperature in the sample set and if
the column met basic system suitability requirements. Up to 50
sample sets can be stored on the eCord chip.
V. ADDITIONAL INFORMATION
a. Tips for Maximizing ACQUITY UPLC BEH Column Lifetimes
1. To maximize ACQUITY UPLC column lifetime, pay close
attention to:
■ ■
Water quality (including water purification system)
■ ■
Solvent quality
■ ■
Mobile-phase preparation, storage and age
■ ■
Sample, buffer and mobile-phase solubilities
■ ■
Sample quality and preparation.
2. When problems arise, often only one improper practice must
be changed.
3. Always remember to:
■ ■
Use in-line filter unit or, preferably, a VanGuard
Pre-Column.
■ ■
Discourage bacterial growth by minimizing the use of
100% aqueous mobile phases where possible.
■ ■
Change aqueous mobile phase every 24 – 48 hours (if
100% aqueous mobile phase use is required).
■ ■
Discard old 100% aqueous mobile phases every 24-48
hours to discourage bacterial growth.
■ ■
Add 5%-10% organic modifier to mobile phase A and
adjust gradient profile.
■ ■
Filter aqueous portions of mobile phase through 0.2 µm
filter.
■ ■
Maintain your water purification system so that it is in good
working order.
■ ■
Only use ultra pure water (18 megohm-cm) water and
highest quality solvents possible. HPLC grade water is not
UPLC grade water.
■ ■
Consider sample preparation (e.g., solid-phase extraction,
filtration, etc).
The eCord chip provides the
user with an overview of the
bulk material QC test results.
The eCord chip provides the
user with QC test conditions
and results on the column
run by the manufacturer. The
information includes mobile
phases, running conditions
and analytes used to test
the columns. In addition the
QC results and acceptance is
placed onto the column.
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[ CARE AND USE MANUAL ]
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