Waters ACQUITY UPLC BEH Columns - Manual (Page 7)

b. Storage
For periods longer than four days at room temperature, store
reversed-phase columns in 100% acetonitrile. For elevated
temperature applications, store immediately after use in 100%
acetonitrile for the best column lifetime. Do not store columns in
buffered eluents. If the mobile phase contained a buffer salt, flush
reversed-phase ACQUITY UPLC BEH Columns with 10 column
volumes of HPLC grade water (see Table 1 for common column
volumes) and replace with 100% acetonitrile for storage. Failure
to perform this intermediate step could result in precipitation
of the buffer salt in the column when 100% acetonitrile is
introduced. Run a gradient to 100% ACN in order to flush all
aqueous solvent from an ACQUITY UPLC BEH Amide Column
prior to storage in 100% ACN. Completely seal column to avoid
evaporation and drying out of the bed.
For periods longer than four days, store ACQUITY UPLC BEH
HILIC Columns in 95:5 acetonitrile:water. Do not store in buffered
solvent. If the mobile phase contained a buffered salt, flush the
column with 10 column volumes of 95:5 acetonitrile:water (see
Table 1 for common column volumes).
Note: If a column has been run with a mobile phase that contains
formate (e.g., ammonium formate, formic acid, etc.) and is then
flushed with 100% acetonitrile, slightly longer equilibration times
may be necessary when the column is re-installed and run again
with a formate-containing mobile phase.
IV. INTRODUCING eCORD INTELLIGENT
CHIP T ECHNOLOGY
a. Introduction
The eCord Intelligent Chip is a new technology that will provide
the history of a column’s performance throughout its lifetime. The
eCord will be permanently attached to the column to assure that
the column’s performance history is maintained in the event that
the column is moved from one instrument to another.
Figure 1. Waters eCord Intelligent Chip
At the time of manufacture, tracking and quality control
information will be downloaded to the eCord. Storing this
information on the chip will eliminate the need for a paper
Certificate of Analysis. Once the user installs the column, the
software will automatically download key parameters into a
column history file stored on the chip. In this manual, we explain
how the eCord will provide a solution for easily tracking the
history of the columns, reduce the frustration of paperwork trails,
and give customers the reassurance that a well-performing column
is installed onto their instruments.
Figure 2. eCord Inserted into Side of Column Heater
b. Installation
Install the column into the column heater. Plug the eCord into
the side of the column heater. Once the eCord is inserted into
the column heater the identification and overall column usage
information will be available in Empower
® and MassLynx ®
software allowing the user to access column information on
their desktop.
Waters eCord intelligent chip
eCord inserted into
side of column heater
7
[ CARE AND USE MANUAL ]
Footer Title RIGHT