Waters BioSuite Columns - Manual (Page 8)

[ Care and Use ManUal ]
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BioSuite Columns 8
C) 30% acetonitrile, 30% methanol, or 30% ethanol in HPLC
grade water, or
D) 8M Urea, 6M Guanidine or other nonionic surfactant HPLC
grade water
Clean the column regularly by injecting up to one column volume
of solution (A) (i.e., 0.1 - 0.2M NaOH in HPLC grade water) in 250
microliter to 2 mL increments. To maximize cleaning effect, reverse the
direction of eluent flow through the column and reduce the flow rate
by 25%.
If desired, the HPLC column can also be cleaned by delivering, in
reverse eluent flow direction and at a 25% standard flow rate, one of
the alternative cleaning solvents (i.e., 20 - 40% acetic acid in HPLC
grade water, or 30% acetonitrile, 30% methanol, or 30% ethanol in
HPLC grade water, or either 8M Urea, 6M Guanidine or other nonionic
surfactant in HPLC grade water).
8. Recommended Storage: For overnight storage, continuously flush the
column with the mobile phase at 10 - 20% of the maximum recom-
mended flow rate. Store the column in the HPLC grade water when it
will be used within 24 hrs or in 20% acetonitrile in HPLC grade water
for long term storage.
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Possible, and BioSuite are trademarks of Waters Corporation.
PEEK is a trademark of Victrex plc.
March 2009 715000652 Rev C KK-PDF
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