Waters BioSuite Columns - Manual (Page 4)

[ Care and Use ManUal ]
BioSuite Columns 4
IV. Waters BIosuIte Phenyl hIc columns
This section contains recommended operating conditions and specifica-
tions for the Waters polymer-based, Phenyl Hydrophobic-interaction
Chromatography columns.
Operating Conditions
1. Shipping Solvent: HPLC grade Water
2. Recommended Operating Flow Rates/Max Pressure:
Note: When using a high viscosity buffer and ambient temperature, the maxi-
mum flow rate may have to be reduced to avoid exceeding the maximum
recommended operating pressure for the column. When changing solvents,
use 50% of the maximum flow rate (20% for the 21.5 mm i.d. preparative
column).
3. Recommended pH Range: 2 - 12 (Avoid extended operation at pH > 10
to maximize column life.)
4. Recommended Organic Conc.: less than 50% (Note: Reduced organic
concentrations may be required if buffers are present in the eluent in
order to avoid salt precipitation.)
5. Recommended Salt Conc.: Less than or equal to 4.0 Molar
6. Recommended Temperature: 10-50 °C. Reduce flow rate when
operating at low temperatures (e.g. 10 °C) to avoid excessive column
pressure.
7. Recommended Cleaning Solvents/Procedures:
A) 0.1 - 0.2M NaOH, and, if not successful,
B) 20 - 40% acetic acid in HPLC grade water.
Clean the column regularly by injecting up to one column volume 0.1
- 0.2M NaOH in 250 µl increments. Use proportionally larger volumes
for the 21.5 mm i.d. preparative column. Following use of cleaning
solvent, rinse the column with 3 to 5 column volumes of HPLC grade
water before storage.
8. Recommended Storage: For overnight storage, continuously flush the
column with the mobile phase at 10 - 20% of the maximum recom-
mended flow rate. Store the column in the HPLC grade water
when it will be used within 24 hrs or in either 20% ethanol or metha-
nol for long term storage. (Caution: Rinse column in HPLC
grade water before switching to 20% organic solvent to avoid salt
precipitation in column.)
V. Waters BIosuIte ultra-hIgh resolutIon (uhr), hIgh
resolutIon (hr), and sIze-exclusIon (sec) columns
This section contains recommended operating conditions and specifications
for the Waters silica-based, ultra-high Resolution (UHR), high-resolution (HR),
and standard size-exclusion (SEC) columns.
Operating Conditions
1. Shipping Solvent: 0.05% Sodium Azide or 0.1M Sodium Sulphate in
0.1M phosphate buffer, pH 6.7
2. Recommended Operating Flow Rate/Max Pressure:
Description Matrix i.d.
(mm)
L
(mm)
Flow Rates /
Max Pressure
BioSuite Phenyl, 1000,
10 µm HIC polymer 7.5 75 0.5 - 1.2 mL/min /
300 PSI
BioSuite Phenyl, 1000,
13 µm HIC polymer 21.5 150 4.0 - 8.0 mL/min /
300 PSI
Description Matrix i.d.
(mm)
L
(mm)
Flow Rates /
Max Pressure
BioSuite 125, 4 µm
UHR SEC silica 4.6 300 0.1 - 0.4 mL/min /
1800 PSI
BioSuite 250, 4 µm
UHR SEC silica 4.6 300 0.1 - 0.4 mL/min /
1800 PSI
BioSuite UHR Guard SEC silica 4.6 35 0.1 - 0.4 mL/min /
1800 PSI
BioSuite 125, 5 µm
HR SEC silica 7.8 300 0.5 - 1.2 mL/min /
1050 PSI
BioSuite 250, 5 µm
HR SEC silica 7.8 300 0.5 - 1.2 mL/min /
1050 PSI
BioSuite 450, 8 µm
HR SEC silica 7.8 300 0.5 - 1.2 mL/min /
525 PSI
BioSuite HR Guard SEC silica 6 40 0.5 - 1.2 mL/min /
1050 PSI
BioSuite 125, 10 µm
SEC silica 7.5 300 0.5 - 1.2 mL/min /
300 PSI
BioSuite 125, 13 µm
SEC silica 21.5 300 3.0 - 8.0 mL/min /
150 PSI
BioSuite 250, 10 µm
SEC silica 7.5 300 0.5 - 1.2 mL/min /
375 PSI