Waters BioSuite Columns - Manual (Page 7)

[ Care and Use ManUal ]
BioSuite Columns 7
6. Recommended Temperature: 4 - 60 °C. Reduce flow rate when
operating at low temperatures (e.g., 4 °C) to avoid excessive column
pressure.
7. Recommended Cleaning Solvents/Procedures:
A) 0.1 - 0.2M NaOH in HPLC grade water and if not successful,
B) 20 - 40% acetic acid in HPLC grade water or,
C) 30% acetonitrile, 30% methanol, or 30% ethanol in HPLC
grade water or,
D) 8M Urea, 6M Guanidine or other nonionic surfactant HPLC
grade water
Clean the column regularly by injecting up to one column volume of
solution (A) (i.e., 0.1 - 0.2M NaOH in HPLC grade water) in 100 to
250 microliter increments. To maximize cleaning effect, reverse the
direction of eluent flow through the column and reduce the flow rate by
25%.
If desired, the HPLC column can also be cleaned by delivering, in
reverse eluent flow direction and at a 25% standard flow rate, one of
the alternative cleaning solvents (i.e., 20 - 40% acetic acid in HPLC
grade water, or 30% acetonitrile, 30% methanol, or 30% ethanol in
HPLC grade water, or either 8M Urea, 6M Guanidine or other nonionic
surfactant in HPLC grade water).
8. Recommended Storage: For overnight storage, continuously flush the
column with the mobilephase at 10 - 20% of the maximum recom-
mended flow rate. Store the column in the HPLC grade water when it
will be used within 24 hrs or in 20% acetonitrile in HPLC grade water
for long term storage.
VIII. Waters BIosuIte Q, deae, sP, and cm columns
This section contains recommended operating conditions and specifications
for the Waters polymer-based, anion- and cation-exchange columns.
Operating Conditions:
1. Shipping Solvent: HPLC grade Water
2. Recommended Operating Flow Rate/Max Pressure:
Note: When using a high viscosity buffer and ambient temperature, the maxi-
mum flow rate may have to be reduced to avoid exceeding the maximum
recommended operating pressure for the column. When changing solvents,
use 50% of the maximum flow rate (20% for the 21.5 mm i.d. preparative
column).
3. Recommended pH Range: 2 - 12 (Avoid extended operation at pH
values less than 3 or greater than 10 to maximize column life.)
4. Recommended Salt Conc.: Less than or equal to 1.0 Molar
5. Recommended Organic Conc.: 0 - 20%.
6. Recommended Temperature: 4 - 40 °C. Reduce flow rate when
operating at low temperatures (e.g., 4 °C) to avoid excessive column
pressure.
7. Recommended Cleaning Solvents/Procedures:
A) 0.1 - 0.2M NaOH in HPLC grade water and if not successful,
B) 20 - 40% acetic acid in HPLC grade water, or
Description Matrix i.d.
(mm)
L
(mm)
Flow Rates /
Max Pressure
BioSuite Q 10 µm AXC polymer 7.5 75 0.5 - 1.2 mL/min /
300 PSI
BioSuite Q 13 µm AXC polymer 21.5 150 4.0 - 8.0 mL/min /
300 PSI
BioSuite DEAE 10 µm
AXC polymer 7.5 75 0.5 - 1.2 mL/min /
225 PSI
BioSuite DEAE 13 µm
AXC polymer 21.5 150 4.0 - 8.0 mL/min /
375 PSI
BioSuite SP 10 µm CXC polymer 7.5 75 0.5 - 1.2 mL/min /
225 PSI
BioSuite SP 13 µm CXC polymer 21.5 150 40 - 8.0 mL/min /
375 PSI
BioSuite CM 10 µm CXC polymer 7.5 75 0.5 - 1.2 mL/min /
225 PSI
BioSuite CM 13 µm CXC polymer 21.5 150 4.0 - 8.0 mL/min /
375 PSI