Waters Dextro-Pak Cartridge - Manual (Page 7)

[ Care and Use ManUal ]
Dextro-Pak Cartridge 7
Table Troubleshooting Procedures
XI. warrant y
Waters warrants its high-performance liquid chromatography
cartridges in accordance with the following terms and conditions:
Waters will repack or replace (at our discretion) without cost, any
cartridge that fails to perform satisfactorily if notified within 60 days
from receipt of the cartridge. Any cartridge returned must have a
prior Return Authorization Number assigned by the Waters Customer
Service Department. Approval is subject to the following exclusions:
• Physical damage to the cartridge because of misuse or abuse.
• Chemical damage to the packing material because of use with
incompatible solvents or buffers, or at an incorrect pH.
• Physical damage to the packing material because of opera-
tion at incorrect temperatures or pressures.
• High internal pressures which have occurred in the cartridge
due to the improper solvent or sample filtration practices
causing particulate buildup or precipitation in the cartridge or
end fittings due to high internal pressures which have occurred
because of improper solvent or sample filtration practices.
XII. orderIng InformatIon
Problem Cause Solution
Excess pressure buildup In-line filters lugged with particulates Replace the in-line filter or shut off the pump. When inlet pressure has
dropped to zero, disconnect the in-line filter from the cartridge and
backflush the prefilter only with mobile phase at 9 ml/min until the
particulates are backflushed out.
Excess pressure buildup Guard column clogged with particles or adsorbed materials Replace spent guard column with fresh packing.
Fluctuating backpressure Cartridge inlet frit plugged with particulates. Always use an in-line filter or guard column to prevent this. Reverse
cartridge to try to wash out particulates at normal flow rate.
Gas in mobile phase Check degassing procedure.
“Spurious” peaks, not due to sugars Faulty pump operation Elution of salts and/or acids Keep mobile phase heated to 70 °C. Inspect pump check valves and
carry out “Ramp Test” (consult the operator’s manual for your pump).
Check sample cleanup procedures.
Leaking mobile phase Fittings in bad state Tighten fittings properly.
Variable elution times of repair Variations in flow rate DO NOT OVERTIGHTEN. Replace worn fittings,o-rings, or seals. Check
system for faults, leaks, especially pulsations from pump.
Band broadening or loss of resolution Guard column fouled Replace spent guard column with fresh packing.
Analytical cartridge fouled or old Try washing with 20% acetonitrile, 80% water. If cartridge does not
recover, use a new cartridge.
Item Part Number
Compression screws and ferrules (5 pk) WAT025604
Guard-Pak~” Inserts Resolve”’ C1e (10 pk) WAT085824