Waters Dextro-Pak Cartridge - Manual (Page 3)

[ Care and Use ManUal ]
Dextro-Pak Cartridge 3
Installation
Note: Follow storage, handling and operation procedures carefully
to obtain results comparable to Waters performance specifications.
Use the Dextro-Pak cartridge with a Waters 8x10 Holder (P/N
WAT082887).
Read each of the two sections on solvent preparation and sample
filtering carefully. Follow the procedures as explained before
performing any separations.
Perform and permanently record the test procedures on your new
column to serve as a performance standard.
1 Cheetham, N.W.H., Sirimanne, P. and Day, W.R., (1981). High-
performance Liquid Chromatographic Separation of Carbohydrate
Oligomers. Journal of Chromatography, 207, 439-444.
II. Column CondItIonIng
Unlike steel columns (which are packed in solvent), radially
compressed cartridges are supplied dry. Before use, the cartridge
must be wetted with a solvent such as methanol or acetonitrile. To
provide a suitable surface for adsorption of the sample:
1. Place a new cartridge into the 8x10 Holder.
2. Prepare solvents as described in the following section before
using them to condition your column.
3. Pump about 50 mL of methanol or acetonitrile through the
cartridge at a flow rate of 1 mL/min.
4. Flush the system thoroughly with water. The cartridge is now
ready for use.
If the cartridge is kept wet, this wetting operation need only be
carried out the first time a cartridge is used.
III. solv ent preparatIon
1. The recommended mobile phase for all analyses is 100%
particulate-free water.
2. Use vacuum filtration through a membrane of 0.45 µm porosity.
Waters Solvent Clarification Kit, P/N WAT085122, is recom-
mended. Suitable filters are Triton-Free Aqueous Replacement
Filters, P/N WAT200538.
3. Remove most dissolved gases using a vacuum filtration of 635
mm (25-inch). Some gases will redissolve from the air and
some growth of microorganisms will occur over a period of time,
even in purified water. Avoid long term problems by heating
the mobile phase in a 1 or 2 liter Erlenmeyer flask on a stirrer/
hotplate. Heat the water to a boil that cannot be stirred down,
then switch off the stirrer and reduce the heat to approximately
70 °C. Cover the neck of the flask with a piece of aluminum foil
to help minimize evaporation.
4. Add fresh mobile phase prepared as above when needed to
allow the system to run continuously.
Detector stability will not be affected because the low flow rate
enables the mobile phase to cool down in the solvent supply line
before it reaches the pump.
Caution: The use of salts and solvent as additives in the mobile phase
will impair cartridge performance and is not recommended.