Waters Dextro-Pak Cartridge - Manual (Page 2)

[ Care and Use ManUal ]
Dextro-Pak Cartridge 2
In sugar analyses, the smallest sugars elute first and usually, as retention time increases, the molecular weight of the sample increases. The
table below shows typical retention times of some carbohydrates on the Dextro-Pak cartridge using water at 1 mL/min as the mobile phase.
These retention times are to be used only as a guide an are not absolute values. Flow rate, cartridge age and cartridge condition may affect the
retention time of the analysis.
Figure 1. Different glucose syrups separated on a Dextro-Pak cartridge
Column: Dextro-Pak, 10 µm, 8 mm x 100 mm
Mobile phase: Water
Flow rate: 1mL/min
Detector: M410, R1, X18
42DE-Acid Digest
62DE-Acid Digest
High DP-Enzyme Digest
High Maltose-Enzyme Digest
Table 1. Typical Retention Times
Compound Retention Time (min) Compound Retention Time (min)
Adonitol 2.87 Maltotriose 4.40/4.75 (doublet)
Arabinose 3.00 Mannitol 2.87
Cellobiose 3.70 Mannose 2.87
2-Deoxyglucose 3.42 Melibiose 3.12
Ethanol 6.83 Methanol 3.90
Ethylene Glycol 3.50 Panose 4.95
Fructose 2.92 Raffi nose 7.00
Fucose 4.07 Rham nose 3.27
Galactose 2.77 Ribose 3.07
Glucose 2.82 Sorbitol 2.85
Glucose-6-phosphate 2.10 Sorbose 2.82
Glycerol 3.15 Stachyose 6.25
lactu lose 3.82 Sucrose 4.37
Lactose 3.00/3.22 (doublet)* Xylitol 2.87
Lycose 2.97 Xylose 2.82 (doublet)*
Maltose 3.45 Xylulose 3.12
* Some sugars such as lactose, xylose and glucose oligomers above a DP of 2, produce two partly resolved peaks
which are attributable to partial separation of anomers.