Waters Styragel Columns - Manual (Page 7)

[ Care and Use ManUal ]
Styragel Columns 7
v. care and maIntenance
This chapter describes:
• Troubleshooting
• Storing the column
• Efficiency testing
a. Troubleshooting
Table 3 describes specific problems, causes, and corrective actions.
Styragel ® HT
Molecular W
eight
Styragel ® HT 2
Elution Volume (mL)
Styragel ® HT 3
Styragel ® HT 4
Styragel ® HT 5
Styragel ® HT 6
Styragel ® HT 6E
Styragel ® HT
Molecular W
eight
Styragel ® HT 2
Elution Volume (mL)
Styragel ® HT 3
Styragel ® HT 4
Styragel ® HT 5
Styragel ® HT 6
Styragel ® HT 6E
Figure 4. Calibration Curves for Waters Styragel HMW Columns
Styragel ® HMW
Molecular W
eight
Styragel ® HMW 2
Elution Volume (mL)
Styragel ® HMW 6E
Styragel ® HMW 7
Styragel ® HMW
Molecular W
eight
Styragel ® HMW 2
Elution Volume (mL)
Styragel ® HMW 6E
Styragel ® HMW 7
Problem Cause Corrective Action
Buildup in system operating
pressure
Inlet filter insert (from the first column only) plugged with par-
ticulates Injector and pump seal shedding
Replace the inlet filter insert.
Install a Styragel Guard column.
Fit an in-line filter between the pump and the
first column.
Clogged tubing Replace the tubing
Loss of resolution, broad peaks,
low plate counts
Filter inserts partially blocked
Replace or clean inlet
and/or outlet filter inserts
Failing injector Repair the injector
Insufficient equilibrium Continue equilibrium
Column damaged Replace column
Table 3. Column Troubleshooting
Figure 3. Calibration Curves for Waters Styragel HT Columns