Waters Styragel Columns - Manual

[ Care and Use ManUal ]
Styragel Columns 1
styragel column
contents
I. IntroductIon
a. Styragel HR
b. Styragel HT
c. Styragel HMW
II. InstallIng the column Bank
a. Preparing the GPC/HPLC System
b. Installing the Columns
c. Repairing Damaged Compression Screw Assemblies
d. Equilibrating the Column Bank
III. PreParIng solvent and samPles
a. Preparing the Solvent
b. Changing Solvents
c. Preparing the Sample
Iv. usIng the column
a. Chromatography Guidelines
b. Calibrating the Column
v. care and maIntenance
a. Troubleshooting
b. Storing the Column
c. Efficiency Testing
1. Testing Instrument Band Spreading
2. Column Efficiency Test
I. IntroductIon
This manual covers the care and use of the Waters Styragel ® HR, HT, and HMW
families of Gel Permeation Chromatography (GPC) columns. Please take a few
moments to read this manual carefully. Follow the recommendations in this
manual to prolong column life and enhance chromatographic reproducibility.
This introduction describes the three families of Waters Styragel columns:
• Styragel HR
• Styragel HT
• Styragel HMW
Waters Styragel columns are packed with high-performance, fully porous, highly
cross-linked styrene-divinylbenzene copolymer particles. Their different charac-
teristics allow you to choose the column optimally suited to your application.
Styragel columns are shipped in three solvents: toluene, tetrahydrofuran (THF),
and dimethylformamide (DMF).