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Care and use manual – Waters Styragel Columns User Manual

Page 10

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[ Care and Use ManUal ]

Styragel Columns

10

Table 6. Conditions and sample for 7.8 mm for Column Efficiency Test:
HT Columns1

Table 7. Conditions and sample for 4.6 mm for Column Efficiency Test:
HT Columns1

Figure 6. Tangent Method for Calculating Column Efficiency

N = 16 (tR/w)

2

tR = Elution distance from injection point to the peak

apex on the chart paper.
W= Peak width of marker measured where tangents
drawn on peak intersect baseline (same units as tRj.

Column Type

Toluene

THF

DMF

Flow Rate

(mL/min)

HT 3

ODCB

Acetone

Acetone

2.0

HT 4

ODCB

Acetone

Acetone

2.0

HT 5

ODCB

Acetone

Acetone

2.0

HT 6

DCHP

Acetone

Acetone

2.0

HT 6E

DCHP

Acetone

Acetone

2.0

Column

Type

Toluene

THF

DMF

Marker

Flow Rate

(mL/min)

Marker

Flow Rate

(mL/min)

Marker

Flow Rate

(mL/min)

HR 3

ODCB

0.3

Acetone

0.3

Acetone

0.3

HR 4

ODCB

0.3

Acetone

0.3

Acetone

0.3

HR 5

ODCB

0.3

Acetone

0.3

Acetone

0.3

HR 6

DCHP

0.3

Acetone

0.3

Acetone

0.3

HR 6E

DCHP

0.3

Acetone

0.3

Acetone

0.3

Table 8. Test Condition and Sample for Efficiency Test 7.8 mm HMW
Columns

Column Type

Toluene

THF

DMF

Flow Rate
(mL/min)

HMW 7

DCHP

Acetone

Acetone

2.0

HMW 6E

DCHP

Acetone

Acetone

2.0