Waters Amino Acid Analysis Liquid Chromatography Column - Manual (Page 8)

[ Care and Use ManUal ]
8
b. column hookup
1. Direction of flow is indicated on the column.
2. Connect one adaptor to the inlet and one adaptor to the outlet of the
column.
3. Connect the tubing from the injector to the column inlet union.
Be sure to use 0.009-0.010 inch (0.23 mm) i.d. tubing for this
purpose.
4. Connect the outlet adaptor to Mixing Tee #1 of the post column
reaction system.
c. storage
If you do not intend to use this column for a period of a week or
longer, it is best to store it in 0.2 N sodium hydroxide (NaOH). Using
your HPLC, pump the filtered NaOH solution through the column at
0.4 ml/min for at least 30 min. To reduce solution viscosity, column
heater should be on at normal analysis temperature (60°-70°C). At
the end of this period turn off pump and disconnect inlet and outlet
tubing. Replace the plug fittings on the column, screwing them in
tightly to prevent evaporation and drying out of the resin.
The HPLC should now be flushed out with filtered water before it is
stored or used for any other pur¬pose. A stainless steel “jumper
tube” which takes the place of the column will prove useful for this
purpose.
appendIx a: amIno acId analysIs column
specIfIcatIons
Column Size
Length: 25 cm
Diameter: 0.46 cm i.d.
Packing Material
Styrene - divinyl benzene copolymer with sulfonic acid
functionality in Na+ form
Particle Size
9 ± 0.5 Nm in diameter
Separation Method
Strong Cation Exchange
pH Range
1-14
Pressure Limit
3000 psi
Shipping Solvent
0.2 M NaOH (Na
+ form)