Waters Sugar-Pak I Column - Manual

[ Care and Use ManUal ]
Sugar-Pak I Columns 1
Contents
I. IntRoDUCtIon
II. PRePARAtIon FoR oPeRAtIon
a. Column Installation
b. Mobile-Phase Requirements
c. Equilibration
III. CARe AnD Use
a. Precautions
b. Storage Considerations (more than 72 hours without use)
c. Starting Up After a Shutdown
d. Overnight Shutdown (less than 72 hours without use)
e. Starting Up After an Overnight Shutdown
IV. sAMPLe PRePARAtIon
a. Pre-Injection Filtration
b. In-Line Filtration
c. Chemical Cleanup of Samples
d. Pre-Injection Cleanup of Proteins and Lipids
e. In-Line Cleanup of Proteins and Lipids
f. Cleanup of Polysacchacrides
g. Removal of Salts and Acids
h. In-Line Cleanup for Samples Not Prone to Hydrolysis
V. CoLUMn ReGeneRAtIon PRoCeDURe
VI. test ConDItIons
VII. tRoUBLesHootInG
VIII. oRDeRInG InFoRMAtIon
SUGAR-PAK I COLUMN
I. Int RoDUCtIon
The Waters Sugar-Pak ™ Column is used for the analysis of sugar
products and process streams in beet, cane, and starch hydrolysis
processing plants; and for incoming quality inspection of commercial
sugar products. Glucose, fructose, maltose, and maltotriose can be
separated from the higher oligomers found in typical corn syrups. The
course of alcoholic fermentations can be followed by monitoring the
reduction of fermentable sugars and the production of alcohol.
The column can be used to separate many monosaccharides,
polyols, and alcohols. Disaccharides and larger sugars are also
separated, mainly according to molecular weight. Many other
useful separations can also be accomplished (e.g., fruit juices,
non-nutritive sweeteners containing sorbitol or mannitol, cell, and
cell hydrolyzates, etc.) where a variety of monosaccharides or sugar
alcohols require separation and analysis.
The 300 x 6.5 mm (ID) Sugar-Pak I Column is packed with a
microparticulate cation-exchange gel in calcium form. To obtain
results comparable to Waters performance specifications, certain
procedures regarding storage, handling, and operation should be
followed carefully, and as explained in this manual.