Waters Protein-Pak HR Ion-Exchange Glass Columns - Manual (Page 8)

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Protein-Pak HR Ion-Exchanged Glass Columns 8
Column: Protein-Pak DEAE 8HR AP
1. Adenosine
2. Carbnic Anhydrase
3. Human Transferrin
4. Ovalbumin
5. Soybean Trypsin Inhibitor
min
Column: Protein-Pak SP 8HR AP-1
1. Myogiobin
2. Ribonuciease A
3. Chymotrypsinogen A
4. Cytochrome C
5. Lysozyme
min
Table 5: Protein Mix for Functional Test Conditions
Figure 2: Typical Chromatograms Using Functional Test Conditions
Mix Amount of Protein
Solution in Mix
Sigma Catalog
Number
Column: Protein-Pak DEAE 8HR AP-1 Protein Mix
Adenosine 30 µL A-9251
Carbonic Anhydras 120 µL C-7500
Human Transferrine 300 µL T-4515
Ovalbumin 240 µL A-7641
Soybean Trypsin Inhibitor 300 µL T-9003
Column: Protein-Pak SP 8HR AP-1 Protein Mix
Myoglobin 50 µL M-0630
Ribonuclease 200 µL R-5000
alpha-Chymotrypsinogen A 100 µL C-4879
Cytochrome C 100 µL C-7752
Lysozyme 100 µL L-6876
Buffer A 200 µL