Waters ACQUITY UPC2 BEH, HSS, and CSH Columns - Manual (Page 5)

5
[ CARE AND USE MANUAL ]
ACQUITY UPC 2 Torus Columns Care & Use
IV. T ROUBLESHOOTING
1. One of the most common problems with regards to columns
is incorrect or insufficient priming of the co-solvent/modifier
pump. If no peaks are observed after an injection or unusually
long retention times are observed, check the priming of the
co-solvent/modifier pump first.
2. If you see frosting on the column at the inlet or outlet, tighten
the compression screw on that side. If tightening doesn’t work,
depressurize the system and the column, and then replace the
fitting that is not sealing correctly.
3. If you continue to see frosting on the column, turn off the CO
2
and vent the system. Allow the column to depressurize fully
before disconnecting the inlet or outlet. Please contact your
Waters representative for additional support.
.
V. COLUMN CLEANING, AGING, REGENERATING,
AND STORAGE
Changes in peak shape, peak splitting, shoulders on the
peak, shifts in retention, change in resolution or increasing
backpressure may indicate contamination of the column.
Flushing with high concentrations of organic solvent, taking
care not to precipitate buffers, is usually sufficient to remove
the contaminant. If the flushing procedure does not solve the
problem, purge the column using the following cleaning and
regeneration procedures.
a. Cleaning
Use the cleaning routine that matches the properties of the
samples and/or what you believe is contaminating the column
(see Table 4). Placing the column on an ACQUITY UPLC
® System
(or HPLC system) will enable the use of a wide range of solvents
and water to improve cleaning. Flush columns with 20 column
Dimension
100% CO
2
60/40 CO
2
/MeOH
Predicted Max Flow Rate
(mL/min)
Predicted Max Pressure
(psi)
Predicted Max Flow Rate
(mL/min)
Predicted Max Pressure
(psi)
2.1 x 50 mm 3.45 5581 2.00 6011
2.1 x 75 mm 2.65 5970 1.35 5938
2.1 x 100 mm 2.05 5969 1.05 6060
2.1 x 150 mm 1.40 5951 0.70 6002
3.0 x 50 mm 4.00 4179 3.60 5946
3.0 x 75 mm 3.75 4897 2.60 5959
3.0 x 100 mm 3.45 5499 2.00 5919
3.0 x 150 mm 2.70 5977 1.40 6023
Dimension
100% CO
2
60/40 CO
2
/MeOH
Predicted Max Flow Rate
(mL/min)
Predicted Max Pressure
(psi)
Predicted Max Flow Rate
(mL/min)
Predicted Max Pressure
(psi)
2.1 x 50 mm 4.00 3549 4.00 4963
2.1 x 75 mm 4.00 4096 3.75 5973
2.1 x 100 mm 3.85 4530 3.00 5979
2.1 x 150 mm 3.50 5386 2.10 5946
3.0 x 50 mm 4.00 3042 4.00 3703
3.0 x 75 mm 4.00 3275 4.00 4283
3.0 x 100 mm 4.00 3528 4.00 4906
3.0 x 150 mm 4.00 4063 3.80 5965
ACQUITY UPC 2 Columns, 1.7 µm
ACQUITY UPC 2 Columns, 3.5 µm
Table 3. Useable Flow Rate Ranges and Expected ACQUITY UPC 2 System Pressures.