Waters ACQUITY UPC2 BEH, HSS, and CSH Columns - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Safety Considerations
b. Column Connectors and Installation
c. eCord Installation
d. Column Equilibration
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. Solvents
c. Pressure
d. Useable Flow-Rate Ranges and Expected ACQUITY UPC 2
System Pressures
e. Band Spreading Minimization
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, AGING, REGENERATION,
AND STORAGE
a. Cleaning
b. Aging
c. Regeneration
d. Storage
VI. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VII. ACQUIT Y UPC 2 COLUMN FAMILY
ACQUITY UPC 2 BEH, CSH, and HSS Columns
I. INT RODUCTION
Thank you for choosing an ACQUITY UPC 2 ® Column. The ACQUITY
UPC 2 Columns feature Ethylene-Bridged Hybrid (BEH), Charged-
Surface Hybrid (CSH™) and High-Strength Silica (HSS) particle
technologies to provide a wide range of selectivities, excellent peak
shape, and high efficiency. The ACQUITY UPC
2 packing materials
are designed for use with the ACQUITY UPC 2 Systems and are
manufactured in an ISO 9000 certified plant using ultra-pure
reagents. Each batch of ACQUITY UPC
2 material is tested and the
results are held to narrow specification ranges to assure excellent,
reproducible performance. Every column is individually tested and
a Performance Chromatogram and Certificate of Batch Analysis is
provided on the eCord™ Intelligent Chip.
ACQUITY UPC 2 Columns are designed and tested specifically for
use on ACQUITY UPC 2 Systems. ACQUITY UPC 2 Columns will
exhibit maximum chromatographic performance and benefit
ONLY when used on holistically-designed ACQUITY UPC
2 Systems
since these systems and columns were created and designed to
operate together. For these reasons, Waters cannot support the
use of ACQUITY UPC
2 Columns on any system other than an
ACQUITY UPC 2 System.