Waters ACQUITY UPC2 BEH, HSS, and CSH Columns - Manual (Page 3)

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[ CARE AND USE MANUAL ]
ACQUITY UPC 2 Torus Columns Care & Use
2. Determine the number of theoretical plates (N) and use this
value as a benchmark for periodic comparisons.
3. Repeat the test at predetermined intervals to track
column performance over time. (Slight variations may
occur if performance tests are performed on two different
ACQUITY UPC
2 Systems due to the quality of the connections,
operating environment, system electronics, reagent quality
and column condition.)
Empty Column
Volumes (mL)
Column Internal Diameter (mm)
Column Length 2.1 mm 3.0 mm
50 mm 0.2 0.4
75 mm 0.3 0.6
100 mm 0.4 0.8
150 mm 0.5 1.0
Table 2. Empty Column Volumes in mL (multiply by 10 for f lush
solvent volumes).
f. VanGuard Pre-Columns
VanGuard™ Pre-columns are 2.1 mm i.d. x 5 mm length guard
column devices designed specifically for use on the ACQUITY
UPC
2 Systems. VanGuard Pre-columns are packed with the same
chemistries and frits as our 2.1 mm i.d. ACQUITY UPC 2 Columns.
VanGuard Pre-columns are designed to be attached directly to the
inlet side of an ACQUITY UPC
2 Column.
Note: In order to ensure void-free and leak-free connections, the
VanGuard Pre-column is shipped with the collet and ferrule NOT
permanently attached. Care must be taken when removing the
O-ring that holds these two pieces on the pre-column tubing.
Figure 1. Connecting a VanGuard Pre-column to an ACQUITY UPC
2 Column.
Installation Instructions
1. Remove VanGuard Pre-column from box and shipping tube and
remove plastic plug.
2. Orient pre-column so that male end is facing up and
carefully remove rubber O-ring that holds collet and ferrule
in place during shipping (collet and ferrule are not yet
permanently attached).
3. Orient ACQUITY UPC 2 Column perpendicular to work surface
so that column inlet is on the bottom (facing down), with
column outlet on top (facing up).
4. From below, insert VanGuard Pre-column into ACQUITY UPC 2
Column inlet and hand-tighten (collet and ferrule are not yet
permanently attached).
5. While pushing the VanGuard Pre-column into the column inlet,
turn assembled column and pre-column 180˚ so that pre-
column is now on top.
6. Tighten with two 5/16” wrenches placed onto ACQUITY UPC 2
Column flats and VanGuard Pre-column hex nut (male end) as
shown above.
7. Tighten 1/4 turn to set collet and ferrule.
8. Check that ferrule is set by loosening connection and
inspecting the ferrule depth. A properly set ferrule depth will
resemble other connections in the ACQUITY UPC
2 System.
9. Reattach pre-column, connect VanGuard Pre-column and
analytical column combination to the ACQUITY UPC 2 System,
apply mobile-phase flow and inspect for leaks.
ACQUITY UPC 2 Column VanGuard Pre-Column
Place wrench here
Ferrule
Collet
Place wrench here
Flow