Waters XSelect CSH XP 2.5 µm Columns - Manual (Page 7)

XSelect CSH XP 2.5 µm Columns 7
[ CARE AND USE MANUAL ]
c. Solvents
To maintain maximum column performance, use high quality HPLC
or MS grade solvents. Filter all aqueous buffers prior to use through
a 0.2 µm filter. Solvents containing suspended particulate materials
will generally clog the outside surface of the inlet of the column.
This may result in higher backpressure or distorted peak shape.
d. Pressure
XSelect CSH XP 2.5 µm Columns are compatible with HPLC, UHPLC and
UPLC pressures. Table 6 depicts the maximum operation pressure.
Table 6: Maximum Operation Pressure
Column ID Pressure Range
2.1 mm 18,000 psi [1034 bar]
3.0 mm 18,000 psi [1034 bar]
4.6 mm 9000 psi [620 bar]
e. Temperature
XSelect CSH XP 2.5 µm Columns can be used up at intermediate
temperatures to enhance selectivity, reduce solvent viscosity and
increase mass transfer rates.
Chemistry
Temperature Limit
Low pH
Temperature Limit
High pH
XSelect CSH C
18
80 °C 45 °C
XSelect CSH Phenyl-Hexyl 80 °C 45 °C
XSelect CSH Fluoro-Phenyl 60 °C 45 °C
Note: Working in combinations of extreme pH, temperature and pressure may
result in reduced column lifetime.
IV. COLUMN CLEANING, REGENERATION AND STORAGE
a. Cleaning and Regeneration
Changes in peak shape, peak splitting, shouldering peaks, shifts
in retention, change in resolution or increasing backpressure may
indicate contamination of the column. Flush with a neat organic
solvent to remove the non-polar contaminant(s), taking care not to
precipitate any buffered mobile-phase components. If this flushing
procedure does not solve the problem, purge the column with the
following cleaning and regeneration procedures.
Use a cleaning routine that matches the properties of the samples,
stationary-phase type (reversed-phase, normal-phase or HILIC) and
will solubilize the suspected contaminate. Flush with 20 column
volumes of solvent at an intermediate temperature of 45 °C. Return
to the initial mobile-phase conditions by reversing the sequence.
If using a reversed-phase column, purge the column with a sequence
of progressively more non-polar solvents (i.e., water-to-methanol-to-
tetrahydrofuran-to-methylene chloride).
If column performance has not improved after regeneration/
cleaning procedures, contact your local Waters representative for
additional support.
b. Storage after Reversed-Phase Use
For periods longer than four days, store the XP 2.5 µm Column in
100% acetonitrile. For separations utilizing elevated temperature,
store immediately after use in 100% acetonitrile. Do not store
columns in buffered eluents. If the mobile phase contained a buffer
salt, flush the column with 10 column volumes of HPLC grade water
(see Table 3 for column volume information) followed by 10 column
volumes of acetonitrile. Failure to perform this intermediate step
could result in precipitation of the buffer salt in the column when
100% acetonitrile is introduced. Completely seal the column to avoid
solvent evaporation and drying out of the chromatographic bed.
Note: If a column has been run with a formate-containing mobile phase
(e.g., ammonium formate, formic acid, etc.) and is purged with 100%
acetonitrile, slightly longer equilibration times may be necessary when the
column is re-installed and re-wetted with that same formate-containing
mobile phase.
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
The eCord Intelligent Chip Technology represents a significant
advancement in column usage tracking management which can be
realized if the column is installed on an ACQUITY UPLC System.
The eCord Intelligent Chip provides a paperless tracking history of the
column’s performance and usage throughout its lifetime. The eCord
is permanently attached to the column body via a tether that cannot
be removed. This ensures that the history of the column is always
accessible to the user of that column.